LRRC14 attenuates Toll-like receptor-mediated NF-κB signaling through disruption of IKK complex

Exp Cell Res. 2016 Sep 10;347(1):65-73. doi: 10.1016/j.yexcr.2016.07.011. Epub 2016 Jul 15.

Abstract

Activation of NF-κB signaling plays pivotal roles in innate immune responses against pathogens. It requires strict control to avert inflammatory diseases. However, the mechanisms underlying this tight regulation are not completely understood. Here, we identified LRRC14, a novel member of LRR (leucine-rich repeat) protein family, as a negative regulator in TLR signaling. Expression of LRRC14 resulted in decreased activation of NF-κB, whereas knockdown of LRRC14 enhanced NF-κB activation as well as the production of inflammatory cytokines. Mechanistically, LRRC14 bound to HLH domain of IKKβ to block its interaction with NEMO and thereby inhibiting the phosphorylation of IKKβ and NF-κB activation. In addition, our data showed that TLR signaling led to lower expression of LRRC14. Together, LRRC14 may function as a checkpoint to prevent overzealous inflammation.

Keywords: IKKβ; Leucine-rich repeat; NF-κB; Phosphorylation; Toll-like receptor.

MeSH terms

  • Animals
  • Cell Line
  • Humans
  • I-kappa B Kinase / metabolism*
  • Leucine-Rich Repeat Proteins
  • Mice
  • NF-kappa B / metabolism*
  • Phosphorylation
  • Protein Binding
  • Proteins / metabolism*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Signal Transduction*
  • Toll-Like Receptors / metabolism*

Substances

  • IKBKG protein, human
  • Leucine-Rich Repeat Proteins
  • NF-kappa B
  • Proteins
  • RNA, Messenger
  • Toll-Like Receptors
  • I-kappa B Kinase