Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD

Mineyuki Mizuguchi; Takayuki Obita; Asagi Kajiyama; Yuki Kozakai; Tsuyoshi Nakai; Yuko Nabeshima; Hitoshi Okazawa

doi:10.1002/1873-3468.12256

Allosteric modulation of the binding affinity between PQBP1 and the spliceosomal protein U5-15kD

FEBS Lett. 2016 Jul;590(14):2221-31. doi: 10.1002/1873-3468.12256. Epub 2016 Jun 28.

Authors

Mineyuki Mizuguchi^{1

2}, Takayuki Obita¹, Asagi Kajiyama¹, Yuki Kozakai¹, Tsuyoshi Nakai¹, Yuko Nabeshima¹, Hitoshi Okazawa^{3

4}

Affiliations

¹ Faculty of Pharmaceutical Sciences, University of Toyama, Japan.
² Graduate School of Innovative Life Science, University of Toyama, Japan.
³ Department of Neuropathology, Medical Research Institute, Tokyo Medical and Dental University, Bunkyo-ku, Japan.
⁴ Center for Brain Integration Research, Tokyo Medical and Dental University, Bunkyo-ku, Japan.

PMID: 27314904
DOI: 10.1002/1873-3468.12256

Abstract

Polyglutamine tract-binding protein 1 (PQBP1) is an intrinsically disordered protein composed of a small folded WW domain and a long disordered region. PQBP1 binds to spliceosomal proteins WBP11 and U5-15kD through its N-terminal WW domain and C-terminal region, respectively. Here, we reveal that the binding between PQBP1 and WBP11 reduces the binding affinity between PQBP1 and U5-15kD. Our results suggest that the interaction between PQBP1 and WBP11 negatively modulates the U5-15kD binding of PQBP1 by an allosteric mechanism.

Keywords: allosteric mechanism; interaction; intrinsically disordered protein.

Publication types

Letter

MeSH terms

Allosteric Regulation / physiology
Carrier Proteins / chemistry*
Carrier Proteins / genetics
Carrier Proteins / metabolism
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Humans
Nuclear Proteins / chemistry*
Nuclear Proteins / genetics
Nuclear Proteins / metabolism
Protein Binding / physiology
Protein Domains
RNA Splicing Factors
Ribonucleoprotein, U5 Small Nuclear / chemistry*
Ribonucleoprotein, U5 Small Nuclear / genetics
Ribonucleoprotein, U5 Small Nuclear / metabolism

Substances

Carrier Proteins
DNA-Binding Proteins
Nuclear Proteins
PQBP1 protein, human
RNA Splicing Factors
Ribonucleoprotein, U5 Small Nuclear
TXNL4A protein, human
WBP11 protein, human