Exploration of Stereoselectivity in Embryo-Larvae (Danio rerio) Induced by Chiral PCB149 at the Bioconcentration and Gene Expression Levels

Tingting Chai; Feng Cui; Xiyan Mu; Yang Yang; Chengju Wang; Jing Qiu

doi:10.1371/journal.pone.0155263

Exploration of Stereoselectivity in Embryo-Larvae (Danio rerio) Induced by Chiral PCB149 at the Bioconcentration and Gene Expression Levels

PLoS One. 2016 May 9;11(5):e0155263. doi: 10.1371/journal.pone.0155263. eCollection 2016.

Authors

Tingting Chai^{1

2}, Feng Cui¹, Xiyan Mu^{1

3}, Yang Yang¹, Chengju Wang¹, Jing Qiu²

Affiliations

¹ College of Science, China Agricultural University, Beijing, China.
² Institute of Quality Standards & Testing Technology for Agro-Products, Key Laboratory of Agro-product Quality and Safety, Chinese Academy of Agricultural Sciences, Key Laboratory of Agri-food Quality and Safety, Ministry of Agriculture, Beijing, China.
³ Center of Fishery Resources and Ecology Environment Research, Chinese Academy of Fishery Sciences, Beijing, China.

Abstract

This paper was designed to study stereoselective enrichment and changes in gene expression when zebrafish (Danio rerio) embryo-larvae were exposed to racemic, (-)- or (+)- PCB149 (2,2',3,4',5',6- hexachlorobiphenyl). Based on bioconcentration analysis, non-racemic enrichment was significantly observed after racemic exposure. No isomerization between the two isomers was found after (-)/(+)-PCB149 exposure. Furthermore, based on gene expression-data mining, CYPs genes (cyp2k6, cyp19a1b, and cyp2aa4) were differential genes after (+)-PCB149 exposure. No obvious differences of dysregulation of gene expression caused by racemic and (-)-PCB149, were observed in embryo-larvae. The above results suggested that (-)-PCB149 could be considered as the main factor causing the dysregulation of gene expression in embryo-larvae after racemic exposure; and (+)-PCB149 should be pursued apart from the racemate, when considering the toxicity of chiral PCB149. Thus, the information in our study could provide new insights to assess the environmental risk of chiral PCBs in aquatic systems.

Publication types

Validation Study

MeSH terms

Animals
Gene Expression / drug effects*
Larva / drug effects*
Polychlorinated Biphenyls / toxicity*
Stereoisomerism
Zebrafish / embryology*
Zebrafish / growth & development

Substances

2,2',3,4',5',6-hexachlorobiphenyl
Polychlorinated Biphenyls

Grants and funding

This work was supported by the National Natural Science Foundation of China (No. 21477161 and 21177156) to JQ. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.