Role of RANKL in the regulation of NFATc1 and c‑Src mRNA expression in osteoclast‑like cells

Mol Med Rep. 2016 Jun;13(6):5163-8. doi: 10.3892/mmr.2016.5176. Epub 2016 Apr 25.

Abstract

This study was designed to determine the effects of receptor activator of nuclear factor κB ligand (RANKL) on the mRNA expression of nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) and c‑Src in rat osteoclast‑like cells. The marrow cells were exposed to macrophage colony-stimulating factor (M‑CSF; 25 ng/ml) and different concentrations of RANKL (0, 50, 75 and 100 ng/ml) for 9 days. The mRNA expression of NFATc1 and c‑Src was determined by polymerase chain reaction. Compared with the M‑CSF (25 ng/ml)+RANKL (0 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression were significantly increased in the M‑CSF (25 ng/ml)+RANKL (75 and 100 ng/ml) groups (P<0.01, P<0.01, P<0.01 and P<0.01, respectively). Compared with the M‑CSF (25 ng/ml)+RANKL (50 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression was significantly increased in the M‑CSF (25 ng/ml)+RANKL (75 and 100 ng/ml) groups (P<0.05, P<0.01, P<0.01 and P<0.01, respectively). Compared with M‑CSF (25 ng/ml)+RANKL (75 ng/ml) group, the levels of NFATc1 and c‑Src mRNA expression was significantly increased in the M‑CSF (25 ng/ml)+RANKL (100 ng/ml) group, (P<0.01 and P<0.01, respectively). These data suggest that RANKL could regulate the expression of NFATc1 and c‑Src mRNA in the marrow culture system.

MeSH terms

  • Animals
  • CSK Tyrosine-Protein Kinase
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Female
  • Gene Expression Regulation, Enzymologic / drug effects*
  • Osteoclasts / cytology
  • Osteoclasts / metabolism*
  • RANK Ligand / pharmacology*
  • Rats
  • Rats, Sprague-Dawley
  • Transcription Factors / metabolism*
  • src-Family Kinases / biosynthesis*

Substances

  • NFATC1 protein, rat
  • RANK Ligand
  • Transcription Factors
  • CSK Tyrosine-Protein Kinase
  • src-Family Kinases