Evidence for decreased expression of APPL1 associated with reduced insulin and adiponectin receptors expression in PCOS patients

J Endocrinol Invest. 2016 Sep;39(9):1075-82. doi: 10.1007/s40618-016-0468-y. Epub 2016 Apr 13.

Abstract

Purpose: To investigate the expression of Adaptor protein containing a PH domain, PTB domain and leucine zipper motif 1 (APPL1), insulin receptor (INSR), adiponectin and adiponectin receptors (adipoR1 and R2) and their possible associations in granulosa cells (GCs) of 22 polycystic ovary syndrome (PCOS) women compared to the 22 non-PCOS controls with normal ovulatory function matched for BMI (body mass index).

Methods: In this study, 44 infertile women aged 18-40 years undergoing in vitro fertilization (IVF) protocol were recruited. After follicular fluid collection, GCs were isolated and then purified with MACS (Micro Beads conjugated to monoclonal anti-human CD45 antibodies). RNA was extracted from GCs and quantitative real-time PCR (qRT-PCR) was performed to assess APPL1 gene expression.

Results: Expression of APPL1, insulin receptor and adiponectin system genes was significantly decreased in PCOS group compared to the controls.

Conclusions: Reduction of APPL1, insulin receptor and adiponectin system genes in GCs could be involved in the development of PCOS.

Keywords: APPL 1; Adiponectin; Adiponectin receptor; Granulosa cell; Insulin receptor; Polycystic ovary syndrome.

Publication types

  • Comparative Study

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics*
  • Adaptor Proteins, Signal Transducing / metabolism
  • Adiponectin / genetics*
  • Adiponectin / metabolism
  • Adolescent
  • Adult
  • Biomarkers / metabolism
  • Case-Control Studies
  • Cells, Cultured
  • Female
  • Granulosa Cells / cytology
  • Granulosa Cells / metabolism
  • Humans
  • Polycystic Ovary Syndrome / genetics*
  • Polycystic Ovary Syndrome / metabolism
  • Polycystic Ovary Syndrome / pathology
  • Real-Time Polymerase Chain Reaction
  • Receptor, Insulin / genetics*
  • Receptor, Insulin / metabolism
  • Receptors, Adiponectin / genetics*
  • Receptors, Adiponectin / metabolism
  • Young Adult

Substances

  • ADIPOQ protein, human
  • APPL1 protein, human
  • Adaptor Proteins, Signal Transducing
  • Adiponectin
  • Biomarkers
  • Receptors, Adiponectin
  • Receptor, Insulin