Objective: To investigate the effect of exogenous small interference RNA(siRNA)-mediated silencing of the cartilage homeoprotein 1 (CART1) gene on cell biological behavior in MDA-MB-231 human breast cancer cells.
Methods: We designed and chemically synthesized siRNA targeting CART1 or control, and then siRNA was transfected into MDA-MB-231 cells using RNAiMAX. The suppression effect of siRNA on CART1 expression was determined by real-time quantitative PCR and Western blotting. The effects of CART1 knockdown on MDA-MB-231 cell invasion, proliferation and cell cycle were detected by TranswellTM invasion assay, CCK-8 assay and flow cytometry, respectively.
Results: CART1-siRNA could significantly suppress the expression of CART1 mRNA and protein as compared with the control group. MDA-MB-231 cells transfected with CART1 siRNA had lower capacity of invasion and proliferation compared with the control group. What's more, the cell ratio increased in S phase and decreased in G2/M in the CART1 siRNA-transfected cells.
Conclusion: Knockdown of the CART1 gene could significantly inhibit cell invasion and proliferation and induce cell cycle arrest in S phase in MDA-MB-231 human breast cancer cells.