Cloning and sequence analysis of the fermentative ...[Gene. 1989]

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1: Gene. 1989 Dec 21;85(1):209-14. Links

Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli.

Goodlove PE, Cunningham PR, Parker J, Clark DP.

Department of Microbiology, Southern Illinois University, Carbondale 62901.

A 6-kb fragment of DNA, which complemented defects in the alcohol dehydrogenase (ADH)-encoding gene (adhE) of Escherichia coli, was cloned into a multicopy vector. Both ADH and coenzyme-A-linked acetaldehyde dehydrogenase (ACDH) activities were encoded by the plasmid, pHIL8. The adhE gene was identified as an open reading frame of 891 codons encoding an Mr 96,008 protein (minus the initiating methionine). Codon usage analysis indicates that adhE should be highly expressed. This gene shows no significant homology to any previously sequenced ADH-encoding gene.

PMID: 2695398 [PubMed - indexed for MEDLINE]

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Cloning and sequence analysis of the fermentative alcohol-dehydrogenase-encoding gene of Escherichia coli.

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