Abstract
We studied the expression of AhR and ATG16L1 protein in experimental oxazolone-induced colitis in rats and anti-inflammatory action of recombinant antagonist of IL-1 receptors (ARIL-1) and simvastatin. The immunopositive cells were determined using an indirect immunofluorescence technique with using a monoclonal rat antibody. It has been established that development of colitis was accompanied by an increase of total number of ATG16L1-lymphocytes (by 30%, P < 0.05) in lymphoid structures of the colon. However the amount of AhR(+)-lymphocytes has not changed. At the same time has increased the concentration of ATG16L1 protein (by 4-11%, P < 0.05) in immunopositive cells. Administration of simvastatin and ARIL-1 during the development of experimental pathology was accompanied by decrease of total number of AhR(+) (by 24-38%, P < 0.05) and ATG16L1(+)-lymphocytes (by 43% - 2 fold, P < 0.05) in the colon.
MeSH terms
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Animals
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Anti-Inflammatory Agents / pharmacology*
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Antibodies, Monoclonal / chemistry
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Autophagy-Related Proteins
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Carrier Proteins / genetics*
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Carrier Proteins / immunology
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Colitis / chemically induced
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Colitis / drug therapy*
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Colitis / immunology
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Colitis / pathology
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Colon / drug effects
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Colon / immunology
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Colon / metabolism*
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Colon / pathology
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Gene Expression Regulation
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Immunophenotyping
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Lymphocyte Count
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Lymphocytes / drug effects
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Lymphocytes / immunology
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Lymphocytes / pathology
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Male
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Oxazolone
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Peptides / metabolism
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Peptides / pharmacology
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Rats
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Rats, Wistar
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Receptors, Aryl Hydrocarbon / genetics*
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Receptors, Aryl Hydrocarbon / immunology
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Receptors, Interleukin-1 / antagonists & inhibitors
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Receptors, Interleukin-1 / genetics
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Receptors, Interleukin-1 / immunology
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Recombinant Proteins / biosynthesis
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Recombinant Proteins / pharmacology
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Simvastatin / pharmacology
Substances
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ATG16L1 protein, human
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Anti-Inflammatory Agents
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Antibodies, Monoclonal
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Autophagy-Related Proteins
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Carrier Proteins
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Peptides
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Receptors, Aryl Hydrocarbon
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Receptors, Interleukin-1
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Recombinant Proteins
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Oxazolone
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Simvastatin