Transducin-like enhancer of split-1 is expressed and functional in human macrophages

Federica De Paoli; Corinne Copin; Jonathan Vanhoutte; Bruno Derudas; Manjula Vinod; Christophe Zawadzki; Sophie Susen; François Pattou; Stéphan Haulon; Bart Staels; Jérome Eeckhoute; Giulia Chinetti-Gbaguidi

doi:10.1002/1873-3468.12029

Transducin-like enhancer of split-1 is expressed and functional in human macrophages

FEBS Lett. 2016 Jan;590(1):43-52. doi: 10.1002/1873-3468.12029. Epub 2015 Dec 30.

Affiliations

¹ Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, University of Lille, France.
² Inserm, CHU Lille, University of Lille, U1190, EGID, France.
³ CHU Lille, France.
⁴ INSERM, U 1081, Institute for Research on Cancer and Aging of Nice (IRCAN), 'Aging and Diabetes' team, University of Nice-Sophia Antipolis, France.
⁵ Clinical Chemistry Laboratory, University Hospital, Nice, France.

PMID: 26763127
DOI: 10.1002/1873-3468.12029

Abstract

Macrophages display heterogeneous phenotypes, including the classical M1 proinflammatory and the alternative M2 anti-inflammatory polarization states. The transducin-like enhancer of split-1 (TLE1) is a transcriptional corepressor whose functions in macrophages have not been studied yet. We report that TLE1 is highly expressed in human alternative macrophages in vitro and in atherosclerotic plaques as well as in adipose tissue M1/M2 mixed macrophages. TLE1 silencing in alternative macrophages decreases the expression of the M2 markers IL-1Ra and IL-10, while it exacerbates TNFα and CCL3 induction by lipopolysaccharide. Hence, TLE1 is expressed in human macrophages where it has potential anti-inflammatory and alternative phenotype promoting properties.

Keywords: atherosclerosis; inflammation; macrophage polarization; transcription factors.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Biomarkers / metabolism
Body Mass Index
Bone Marrow Cells / cytology
Bone Marrow Cells / drug effects
Bone Marrow Cells / immunology
Bone Marrow Cells / metabolism
Cells, Cultured
Co-Repressor Proteins / antagonists & inhibitors
Co-Repressor Proteins / genetics
Co-Repressor Proteins / metabolism*
Female
Gene Expression Regulation / drug effects
Humans
Interleukin 1 Receptor Antagonist Protein / antagonists & inhibitors
Interleukin 1 Receptor Antagonist Protein / genetics
Interleukin 1 Receptor Antagonist Protein / metabolism
Interleukin-10 / antagonists & inhibitors
Interleukin-10 / genetics
Interleukin-10 / metabolism
Interleukin-4 / genetics
Interleukin-4 / metabolism
Interleukin-4 / pharmacology
Intra-Abdominal Fat / immunology
Intra-Abdominal Fat / metabolism
Intra-Abdominal Fat / pathology
Macrophage Activation* / drug effects
Macrophages / drug effects
Macrophages / immunology
Macrophages / metabolism*
Macrophages / pathology
Male
Mice, 129 Strain
Obesity / blood
Obesity / immunology
Obesity / metabolism
Obesity / pathology
Plaque, Atherosclerotic / immunology
Plaque, Atherosclerotic / metabolism
Plaque, Atherosclerotic / pathology
RNA Interference
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Recombinant Proteins / pharmacology
Repressor Proteins / antagonists & inhibitors
Repressor Proteins / genetics
Repressor Proteins / metabolism*

Substances

Biomarkers
Co-Repressor Proteins
IL10 protein, human
IL1RN protein, human
IL4 protein, human
Interleukin 1 Receptor Antagonist Protein
Recombinant Proteins
Repressor Proteins
TLE1 protein, human
Tle1 protein, mouse
Interleukin-10
Interleukin-4