Clinical and prognostic significance of Arl4c expression in colorectal cancer

Cancer Biomark. 2016;16(2):253-7. doi: 10.3233/CBM-150562.

Abstract

Background: ADP-ribosylation factor (ARF)-like 4c (Arl4c) has been reported to promote tumorigenesis in colorectal and lung cancers and may represent a novel therapeutic target. The aim of this study was to investigate the expression of ADP-ribosylation factor (ARF)-like 4c (Arl4c) in colorectal cancer (CRC) in Chinese populations and an AOM/DSS-induced mouse colorectal carcinogenesis model, and its prognostic value in patients with CRC.

Materials and methods: Expression of Arl4c in CRC patients and AOM/DSS-induced colorectal tumors was detected using immunohistochemistry and real- Real time PCR. In addition, the correlations between Arl4c expression and clinicopathological characteristics and overall survival were evaluated using Kaplan-Meier analysis.

Results: The Arl4c was statistically significantly overexpressed in CRC tissues relative to adjacent normal tissue (p= 0.001). CRC patients with high Arl4c expression tended to show poorer survival than patients with lower Arl4c expression (p= 0.069). However, the level of Arl4c expression was not visibly correlated with age, gender or tumor stage. In addition, increased expression of Arl4c was also found in AOM/DSS-induced colorectal tumors (p= 0.001), which indicated that Arl4c might play an important role in colon tumorigenesis.

Conclusions: Results indicated that overexpression of Arl4c might contribute to the tumorigenesis and might play a pivotal role in the progression of CRC.

Keywords: Arl4c; colorectal cancer; prognosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-Ribosylation Factors / genetics*
  • ADP-Ribosylation Factors / metabolism
  • Adult
  • Aged
  • Aged, 80 and over
  • Biomarkers, Tumor*
  • Case-Control Studies
  • Cell Transformation, Neoplastic / genetics
  • Colorectal Neoplasms / diagnosis*
  • Colorectal Neoplasms / genetics*
  • Colorectal Neoplasms / mortality
  • Disease Progression
  • Female
  • Gene Expression
  • Humans
  • Immunohistochemistry
  • Kaplan-Meier Estimate
  • Male
  • Middle Aged
  • Neoplasm Staging
  • Prognosis
  • Real-Time Polymerase Chain Reaction
  • Tumor Burden
  • Young Adult

Substances

  • Biomarkers, Tumor
  • ADP-Ribosylation Factors
  • ARL4C protein, human