Assignment of human desmin gene to band 2q35 by nonradioactive in situ hybridization

Hum Genet. 1989 Aug;83(1):33-6. doi: 10.1007/BF00274143.

Abstract

A 3-kb DNA fragment, inserted in Bluescribe vector, was used to localize the desmin gene by in situ hybridization on human metaphase chromosomes. The probe was labelled by Bio-11-dUTP and detected by immunofluorescence. Subsequent R-banding indicated that the desmin gene is located in band 2q35.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotin
  • Chromosome Mapping / methods*
  • Chromosomes, Human, Pair 2 / ultrastructure*
  • DNA Probes
  • Desmin / genetics*
  • Fluorescent Antibody Technique
  • Humans
  • Nucleic Acid Hybridization*

Substances

  • DNA Probes
  • Desmin
  • Biotin