Abstract
Here, we identify ATP1B3 and fibrillin-1 as novel BST-2-binding proteins. ATP1B3 depletion in HeLa cells (BST-2-positive cells), but not 293T cells (BST-2-negative cells), induced the restriction of HIV-1 production in a BST-2-dependent manner. In contrast, fibrillin-1 knockdown reduced HIV-1 production in 293T and HeLa cells in a BST-2-independent manner. Moreover, NF-κB activation was enhanced by siATP1B3 treatment in HIV-1- and HIV-1ΔVpu-infected HeLa cells. In addition, ATP1B3 silencing induced high level BST-2 expression on the surface of HeLa cells. These results indicate that ATP1B3 is a co-factor that accelerates BST-2 degradation and reduces BST-2-mediated restriction of HIV-1 production and NF-κB activation.
Keywords:
ATP1B3; BST-2; NF-kappa B (NF-KB); Na+/K+-ATPase; bone marrow; cell biology; cell surface protein; human immunodeficiency virus (HIV); membrane trafficking; retrovirus.
© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, CD / chemistry
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Antigens, CD / genetics
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Antigens, CD / metabolism*
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Cell Line
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Fibrillin-1
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Fibrillins
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GPI-Linked Proteins / antagonists & inhibitors
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GPI-Linked Proteins / chemistry
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GPI-Linked Proteins / genetics
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GPI-Linked Proteins / metabolism
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Gene Deletion
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Gene Expression Regulation
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HEK293 Cells
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HIV-1 / physiology*
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HeLa Cells
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Humans
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Microfilament Proteins / antagonists & inhibitors
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Microfilament Proteins / chemistry
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Microfilament Proteins / genetics
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Microfilament Proteins / metabolism*
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NF-kappa B / agonists
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NF-kappa B / antagonists & inhibitors*
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NF-kappa B / genetics
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NF-kappa B / metabolism
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Protein Interaction Domains and Motifs
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Protein Stability
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Proteolysis
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RNA Interference
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / metabolism
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Recombinant Proteins / chemistry
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Recombinant Proteins / metabolism
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Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
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Sodium-Potassium-Exchanging ATPase / chemistry
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Sodium-Potassium-Exchanging ATPase / genetics
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Sodium-Potassium-Exchanging ATPase / metabolism*
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Two-Hybrid System Techniques
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Viral Tropism
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Virus Replication
Substances
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ATP1B3 protein, human
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Antigens, CD
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BST2 protein, human
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FBN1 protein, human
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Fibrillin-1
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Fibrillins
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GPI-Linked Proteins
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Microfilament Proteins
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NF-kappa B
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Recombinant Fusion Proteins
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Recombinant Proteins
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Sodium-Potassium-Exchanging ATPase