Global miRNA Expression Profiling Identifies miR-1290 as Novel Potential oncomiR in Laryngeal Carcinoma

PLoS One. 2015 Dec 22;10(12):e0144924. doi: 10.1371/journal.pone.0144924. eCollection 2015.

Abstract

Background: Laryngeal squamous cell carcinoma (LSCC) is the most common group among head and neck cancers. LSCC is characterized by a high incidence in Europe. With the aim of better understanding its genetic background we performed global miRNA expression profiling of LSCC cell lines and primary specimens. By this approach we identified a cohort of 33 upregulated and 9 downregulated miRNA genes in LSCC as compared to epithelial no tumor controls.

Results: Within this group we identified overexpression of the novel miR-1290 gene not reported in the context of LSCC before. Using a combined bioinformatical approach in connection with functional analysis we delineated two putative target genes of miR-1290 namely ITPR2 and MAF which are significantly downregulated in LSCC. They are interesting candidates for tumor suppressor genes as they are implicated in apoptosis and other processes deregulated in cancer.

Conclusion: Taken together, we propose miR-1290 as the new oncomiR involved in LSCC pathogenesis. Additionally, we suggest that the oncogenic potential of miR-1290 might be expressed by the involvement in downregulation of its target genes MAF and ITPR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions
  • Adult
  • Aged
  • Carcinoma, Squamous Cell / genetics*
  • Cell Line, Tumor
  • Computational Biology / methods
  • Female
  • Gene Expression Profiling / methods*
  • Gene Expression Regulation, Neoplastic
  • Humans
  • Inositol 1,4,5-Trisphosphate Receptors / genetics*
  • Laryngeal Neoplasms / genetics*
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Oligonucleotide Array Sequence Analysis / methods
  • Proto-Oncogene Proteins c-maf / genetics*

Substances

  • 3' Untranslated Regions
  • ITPR2 protein, human
  • Inositol 1,4,5-Trisphosphate Receptors
  • MAF protein, human
  • MIRN1290 microRNA, human
  • MicroRNAs
  • Proto-Oncogene Proteins c-maf

Grants and funding

The study was financed by the Polish National Science Center grant number N N401 195839 (https://www.ncn.gov.pl). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.