Abstract
This study focused on determining the role of Spy1 in human epithelial ovarian cancer (EOC). Speedy is a novel cell cycle protein capable of promoting cell proliferation. In this study, western blot and immunohistochemistrical analyses were performed to detect the expression of Spy1 in ovarian cancer. Spy1 protein levels increased with ovarian cancer grade, and Kaplan-Meier curve showed that overexpression of Spy1 was significantly correlated with reduced patient survival. In vitro, Spy1 depletion in ovarian cell lines led to reduced proliferation according to CCK8 and plate colony assays. The expression of Spy1 was positively related to pThr187-p27. Flow cytometry revealed that the reduced expression of Spy1 induced the apoptosis of the EOC cells. In summary, our findings suggested that Spy1 may be a novel independent prognostic predictor of survival for ovarian patients.
Keywords:
Apoptosis; Ovarian cancer; Proliferation; Spy1.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Apoptosis / genetics
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Biomarkers, Tumor / genetics*
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Biomarkers, Tumor / metabolism
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Carcinoma, Ovarian Epithelial
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Cell Cycle / genetics
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Cell Cycle Proteins / genetics*
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Cell Cycle Proteins / metabolism
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Cell Line, Tumor
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Cell Proliferation
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Female
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Gene Expression
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Humans
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Ki-67 Antigen / genetics
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Ki-67 Antigen / metabolism
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Middle Aged
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Neoplasm Grading
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Neoplasm Proteins / genetics*
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Neoplasm Proteins / metabolism
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Neoplasm Staging
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Neoplasms, Glandular and Epithelial / diagnosis*
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Neoplasms, Glandular and Epithelial / genetics*
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Neoplasms, Glandular and Epithelial / mortality
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Neoplasms, Glandular and Epithelial / pathology
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Ovarian Neoplasms / diagnosis*
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Ovarian Neoplasms / genetics*
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Ovarian Neoplasms / mortality
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Ovarian Neoplasms / pathology
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Ovary / metabolism
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Ovary / pathology
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Prognosis
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Survival Analysis
Substances
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Biomarkers, Tumor
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Cell Cycle Proteins
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Ki-67 Antigen
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Neoplasm Proteins
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SPDYA protein, human