Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis

Atsuko Ikeda; Kentaro Kajiwara; Kunihiko Iwamoto; Asami Makino; Toshihide Kobayashi; Keiko Mizuta; Kouichi Funato

doi:10.1002/yea.3138

Complementation analysis reveals a potential role of human ARV1 in GPI anchor biosynthesis

Yeast. 2016 Feb;33(2):37-42. doi: 10.1002/yea.3138. Epub 2015 Nov 2.

Authors

Atsuko Ikeda¹, Kentaro Kajiwara¹, Kunihiko Iwamoto², Asami Makino², Toshihide Kobayashi², Keiko Mizuta¹, Kouichi Funato¹

Affiliations

¹ Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan.
² Lipid Biology Laboratory and RIKEN, Saitama, Japan.

PMID: 26460143
DOI: 10.1002/yea.3138

Abstract

ARV1 is involved in regulating lipid homeostasis but also in the biosynthesis of glycosylphosphatidylinositol (GPI) in Saccharomyces cerevisiae. Here, we examined whether human ARV1 can complement the role of yeast ARV1 in GPI biosynthesis. Overexpression of human ARV1 could rescue the phenotypes associated with GPI anchor synthesis defect in the yeast arv1Δ mutant. The results suggest that Arv1 function in GPI biosynthesis may be conserved in all eukaryotes, from yeast to humans.

Keywords: GPI anchor; arv1Δ mutant; complementation; human ARV1; yeast.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carrier Proteins / genetics
Carrier Proteins / metabolism*
Gene Expression
Genetic Complementation Test
Glycosylphosphatidylinositols / biosynthesis*
Homeostasis
Humans
Lipid Metabolism
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mutation
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae / metabolism
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism*

Substances

ARV1 protein, S cerevisiae
ARV1 protein, human
Carrier Proteins
Glycosylphosphatidylinositols
Membrane Proteins
Saccharomyces cerevisiae Proteins