MicroRNA-339, an epigenetic modulating target is involved in human gastric carcinogenesis through targeting NOVA1

FEBS Lett. 2015 Oct 7;589(20 Pt B):3205-11. doi: 10.1016/j.febslet.2015.09.009. Epub 2015 Sep 29.

Abstract

The role of miR-339 in human gastric cancer (GC) remains unclear. Here, we found that miR-339 is remarkably decreased in primary GC tissues. Overexpression of miR-339 in GC cells significantly suppressed proliferation, migration, invasion, and tumorigenicity. Furthermore, NOVA1 was confirmed as a target of miR-339. Restoration of NOVA1 in miR-339-overexpressing GC cells partially impaired the inhibitory effects of miR-339. More importantly, epigenetic modification may be involved in the modulation of miR-339 expression. These findings uncover a novel role for miR-339 in gastric carcinogenesis, and restoration of miR-339 could be considered as a potential therapeutic strategy for GC treatment.

Keywords: Gastric cancer; Methylation; NOVA1; Proliferation; miR-339.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3' Untranslated Regions / genetics
  • Animals
  • Base Sequence
  • Cell Line
  • Cell Line, Tumor
  • Cell Movement / genetics
  • Cell Proliferation / genetics
  • DNA Methylation
  • Female
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Kaplan-Meier Estimate
  • Male
  • Mice, Inbred BALB C
  • Mice, Nude
  • MicroRNAs / genetics*
  • Middle Aged
  • Mutation
  • Neuro-Oncological Ventral Antigen
  • RNA-Binding Proteins / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stomach Neoplasms / genetics*
  • Stomach Neoplasms / pathology
  • Transplantation, Heterologous

Substances

  • 3' Untranslated Regions
  • MIRN339 microRNA, human
  • MicroRNAs
  • NOVA1 protein, human
  • Neuro-Oncological Ventral Antigen
  • RNA-Binding Proteins