Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality

Mami Sumiyoshi; Narumi Masuda; Nobuhiro Tanuma; Honami Ogoh; Eri Imai; Mizuki Otsuka; Natsuki Hayakawa; Kinuyo Ohno; Yasuhisa Matsui; Kanae Hara; Risa Gotoh; Mai Suzuki; Shinya Rai; Hirokazu Tanaka; Itaru Matsumura; Hiroshi Shima; Toshio Watanabe

doi:10.1016/j.febslet.2015.07.050

Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit embryonic lethality

FEBS Lett. 2015 Sep 14;589(19 Pt B):2754-62. doi: 10.1016/j.febslet.2015.07.050. Epub 2015 Aug 18.

Authors

Mami Sumiyoshi¹, Narumi Masuda¹, Nobuhiro Tanuma², Honami Ogoh¹, Eri Imai¹, Mizuki Otsuka¹, Natsuki Hayakawa¹, Kinuyo Ohno¹, Yasuhisa Matsui³, Kanae Hara¹, Risa Gotoh¹, Mai Suzuki¹, Shinya Rai⁴, Hirokazu Tanaka⁴, Itaru Matsumura⁴, Hiroshi Shima², Toshio Watanabe⁵

Affiliations

¹ Department of Biological Science, Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan.
² Division of Cancer Chemotherapy, Miyagi Cancer Center Research Institute, Miyagi, Japan.
³ Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University, Miyagi, Japan.
⁴ Department of Hematology and Rheumatology, Kinki University Faculty of Medicine, Osaka, Japan.
⁵ Department of Biological Science, Graduate School of Humanities and Sciences, Nara Women's University, Nara, Japan. Electronic address: toshiwatana@cc.nara-wu.ac.jp.

PMID: 26296315
DOI: 10.1016/j.febslet.2015.07.050

Abstract

In mammals, the small Arf GTPase-activating protein (SMAP) subfamily of Arf GTPase-activating proteins consists of closely related members, SMAP1 and SMAP2. These factors reportedly exert distinct functions in membrane trafficking, as manifested by different phenotypes seen in single knockout mice. The present study investigated whether SMAP proteins interact genetically. We report for the first time that simultaneous loss of SMAP1 and SMAP2 promotes apoptosis in the distal region of E7.5 mouse embryos, likely resulting in embryonic lethality. Thus, at least one SMAP gene, either SMAP1 or SMAP2, is required for proper embryogenesis.

Keywords: Arf GAP; Embryogenesis; Genetic interaction; SMAP1; SMAP2.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Apoptosis
Cell Line
Embryo, Mammalian / cytology
Embryo, Mammalian / embryology*
Embryonic Development
Endocytosis
Humans
Membrane Proteins / deficiency*
Membrane Proteins / genetics
Membrane Proteins / metabolism*
Mice
Protein Transport
Sequence Deletion
Transferrin / metabolism

Substances

Membrane Proteins
SMAP2 protein, mouse
Smap1 protein, mouse
Transferrin