The iPLA(2)γ is identified as the membrane potential sensitive phospholipase in liver mitochondria

Adam J Rauckhorst; Douglas R Pfeiffer; Kimberly M Broekemeier

doi:10.1016/j.febslet.2015.07.016

The iPLA(2)γ is identified as the membrane potential sensitive phospholipase in liver mitochondria

FEBS Lett. 2015 Aug 19;589(18):2367-71. doi: 10.1016/j.febslet.2015.07.016. Epub 2015 Jul 20.

Authors

Adam J Rauckhorst¹, Douglas R Pfeiffer², Kimberly M Broekemeier³

Affiliations

¹ Department of Cellular and Molecular Biochemistry, The Ohio State University, Columbus, OH 43210, United States.
² Department of Cellular and Molecular Biochemistry, The Ohio State University, Columbus, OH 43210, United States; Internal Medicine, The Ohio State University, Columbus, OH 43210, United States; Institute of Mitochondrial Biology, The Ohio State University, Columbus, OH 43210, United States.
³ Department of Chemistry and Biochemistry, Ohio Northern University, Ada, OH 45810, United States. Electronic address: k-broekemeier@onu.edu.

PMID: 26206229
DOI: 10.1016/j.febslet.2015.07.016

Abstract

Previous reports from our lab identified a mitochondrial calcium-independent phospholipase A2 activity that is activated when the mitochondrial membrane potential is decreased. This activity was demonstrated to influence occurrence of the permeability transition. Originally, this activity was ascribed to the iPLA2β protein. Recently, both iPLA2β and iPLA2γ knock out mice have been generated. It has been shown by others that the iPLA2γ plays a significant role in progression of the permeability transition. In this paper, using the iPLA2β and iPLA2γ knock out mice we show that the membrane potential sensitive activity is the iPLA2γ.

Keywords: Bioenergetics; Calcium-independent phospholipase A2; Knockout; Mitochondria; Phospholipase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Enzyme Activation / drug effects
Gene Knockout Techniques
Group VI Phospholipases A2 / antagonists & inhibitors
Group VI Phospholipases A2 / deficiency
Group VI Phospholipases A2 / genetics
Group VI Phospholipases A2 / metabolism*
Male
Membrane Potential, Mitochondrial* / drug effects
Mice
Mitochondria, Liver / metabolism*
Naphthalenes / pharmacology
Pyrones / pharmacology
Rats

Substances

Naphthalenes
Pyrones
6-(bromomethylene)tetrahydro-3-(1-naphthaleneyl)-2H-pyran-2-one
Group VI Phospholipases A2
Pla2g6 protein, mouse