CCR7 is involved in BCR-ABL/STAP-2-mediated cell growth in hematopoietic Ba/F3 cells

Biochem Biophys Res Commun. 2015 Aug 7;463(4):825-31. doi: 10.1016/j.bbrc.2015.06.020. Epub 2015 Jun 21.

Abstract

Chronic myeloid leukemia is a clonal disease characterized by the presence of the Philadelphia chromosome and its oncogenic product, BCR-ABL, which activates multiple pathways involved in cell survival, growth promotion, and disease progression. We previously reported that in murine hematopoietic Ba/F3 cells, signal transducing adaptor protein-2 (STAP-2) binds to BCR-ABL and up-regulates BCR-ABL phosphorylation, leading to enhanced activation of its downstream signaling molecules. The binding of STAP-2 to BCR-ABL also influenced the expression levels of chemokine receptors, such as CXCR4 and CCR7. For the induction of CCR7 expression, signals mediated by the MAPK/ERK pathway were critical in Ba/F3 cells expressing BCR-ABL and STAP-2. In addition, STAP-2 cooperated with BCR-ABL to induce the production of CCR7 ligands, CCL19 and CCL21. Our results demonstrate a contribution of CCR7 to STAP-2-dependent enhancement of BCR-ABL-mediated cell growth in Ba/F3 cells.

Keywords: BCR-ABL; CCR7; CML; MAPK/ERK; STAP-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / physiology*
  • Animals
  • Base Sequence
  • Bone Marrow Cells / cytology*
  • Cell Division / physiology*
  • Cell Line
  • DNA Primers
  • Fusion Proteins, bcr-abl / physiology*
  • Mice
  • Mice, Inbred BALB C
  • Protein Kinases / metabolism
  • Real-Time Polymerase Chain Reaction
  • Receptors, CCR7 / physiology*

Substances

  • Adaptor Proteins, Signal Transducing
  • Ccr7 protein, mouse
  • DNA Primers
  • Receptors, CCR7
  • STAP2 protein, mouse
  • Protein Kinases
  • Fusion Proteins, bcr-abl