Mitochondrial uncoupling protein (UCP2) gene expression is regulated by miR-2909

Blood Cells Mol Dis. 2015 Jun;55(1):89-93. doi: 10.1016/j.bcmd.2015.05.001. Epub 2015 May 5.

Abstract

Reversible decoupling of glycolysis from aerobic-respiration has been widely recognized to be a crucial step in tailoring immune response by the human cells. In this context, the study reported here revealed for the first time that cooperativity between Apoptosis Antagonizing Transcription Factor (AATF) mRNA and miR-2909 within cellular AATF RNome ensures the regulation of mitochondrial uncoupling protein 2 (UCP2) expression in a cyclic fashion and this phenomenon is substantiated when the immune cells face high glucose threat.

Keywords: AATF RNome; CCL5; IL-17; Regulation; UCP2.

MeSH terms

  • Apoptosis Regulatory Proteins / genetics
  • Apoptosis Regulatory Proteins / metabolism*
  • Chemokine CCL5 / genetics
  • Chemokine CCL5 / metabolism
  • Gene Expression Regulation
  • Glucose / metabolism*
  • Glucose / pharmacology
  • HeLa Cells
  • Humans
  • Interleukin-17 / genetics
  • Interleukin-17 / metabolism
  • Ion Channels / genetics
  • Ion Channels / metabolism*
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors / antagonists & inhibitors
  • Kruppel-Like Transcription Factors / genetics
  • Kruppel-Like Transcription Factors / metabolism
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism*
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Mitochondria / drug effects
  • Mitochondria / genetics
  • Mitochondria / immunology
  • Mitochondria / metabolism*
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism*
  • Plasmids / chemistry
  • Plasmids / metabolism
  • Primary Cell Culture
  • Promoter Regions, Genetic
  • Protein Binding
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • Repressor Proteins / genetics
  • Repressor Proteins / metabolism*
  • Signal Transduction
  • Sp1 Transcription Factor / genetics
  • Sp1 Transcription Factor / metabolism
  • Transcription Factor RelA / genetics
  • Transcription Factor RelA / metabolism
  • Transfection
  • Tumor Suppressor Protein p53 / genetics
  • Tumor Suppressor Protein p53 / metabolism
  • Uncoupling Protein 2

Substances

  • AATF protein, human
  • Apoptosis Regulatory Proteins
  • CCL5 protein, human
  • Chemokine CCL5
  • Interleukin-17
  • Ion Channels
  • Kruppel-Like Factor 4
  • Kruppel-Like Transcription Factors
  • MIRN2909 microRNA, human
  • MicroRNAs
  • Mitochondrial Proteins
  • RELA protein, human
  • RNA, Small Interfering
  • Repressor Proteins
  • Sp1 Transcription Factor
  • SP1 protein, human
  • Transcription Factor RelA
  • Tumor Suppressor Protein p53
  • UCP2 protein, human
  • Uncoupling Protein 2
  • Glucose