OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer

Jae-Hwan Kim; Soon-Min Lee; Jong-Hyuk Lee; Sohyun Chun; Byung-Hee Kang; Sojung Kwak; Jae-Seok Roe; Tae Wan Kim; Hyunsoo Kim; Woo Ho Kim; Eun-Jung Cho; Hong-Duk Youn

doi:10.18632/oncotarget.3683

OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer

Oncotarget. 2015 Aug 14;6(23):19528-41. doi: 10.18632/oncotarget.3683.

Authors

Jae-Hwan Kim¹, Soon-Min Lee¹, Jong-Hyuk Lee¹, Sohyun Chun², Byung-Hee Kang¹, Sojung Kwak¹, Jae-Seok Roe¹, Tae Wan Kim¹, Hyunsoo Kim¹, Woo Ho Kim³, Eun-Jung Cho⁴, Hong-Duk Youn^{1

2}

Affiliations

¹ National Creative Research Center for Epigenome Reprogramming Network, Department of Biomedical Sciences, Ischemic/Hypoxic Disease Institute, Seoul National University College of Medicine, Seoul, Republic of Korea.
² Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science, Seoul National University, Seoul, Republic of Korea.
³ Department of Pathology, Seoul National University College of Medicine, Seoul, Republic of Korea.
⁴ College of Pharmacy, Sungkyunkwan University, Suwon, Republic of Korea.

Abstract

2-oxogluatrate and Fe(II)-dependent oxygenase domain-containing protein 1 (OGFOD1) was recently revealed to be a proline hydroxylase of RPS23 for translational termination. However, OGFOD1 is nuclear, whereas translational termination occurs in the cytoplasm, raising the possibility of another function of OGFOD1 in the nucleus. In this study, we demonstrate that OGFOD1 is involved in cell cycle regulation. OGFOD1 knockdown in MDA-MB-231 breast cancer cells significantly impeded cell proliferation and resulted in the accumulation of G1 and G2/M cells by decreasing the mRNA levels of G1/S transition- and G2/M-related transcription factors and their target genes. We also confirmed that OGFOD1 is highly expressed in breast cancer tissues by bioinformatic analysis and immunohistochemistry. Thus, we propose that OGFOD1 is required for breast cancer cell proliferation and is associated with poor prognosis in breast cancer.

Keywords: G2/M phase; OGFOD1; breast cancer; cell cycle.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / genetics
Breast Neoplasms / metabolism*
Breast Neoplasms / mortality
Breast Neoplasms / pathology
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
Cell Proliferation*
Chromatin Assembly and Disassembly
Computational Biology
Databases, Genetic
Female
G1 Phase Cell Cycle Checkpoints
G2 Phase Cell Cycle Checkpoints
Gene Expression Regulation, Neoplastic
Gene Knockdown Techniques
HEK293 Cells
HeLa Cells
Humans
Immunohistochemistry
In Situ Hybridization
Kaplan-Meier Estimate
MCF-7 Cells
Nuclear Proteins / genetics
Nuclear Proteins / metabolism*
Prognosis
RNA Interference
RNA, Messenger / metabolism
Signal Transduction
Time Factors
Transcription Factors / genetics
Transcription Factors / metabolism
Transfection

Substances

Carrier Proteins
Cell Cycle Proteins
Nuclear Proteins
OGFOD1 protein, human
RNA, Messenger
Transcription Factors