Intranuclear dynamics of the Nup107-160 complex

Mol Biol Cell. 2015 Jun 15;26(12):2343-56. doi: 10.1091/mbc.E15-02-0060. Epub 2015 Apr 22.

Abstract

Nup98 is a glycine-leucine-phenylalanine-glycine (GLFG) repeat-containing nucleoporin that, in addition to nuclear transport, contributes to multiple aspects of gene regulation. Previous studies revealed its dynamic localization within intranuclear structures known as GLFG bodies. Here we show that the mammalian Nup107-160 complex (Y-complex), a major scaffold module of the nuclear pore, together with its partner Elys, colocalizes with Nup98 in GLFG bodies. The frequency and size of GLFG bodies vary among HeLa sublines, and we find that an increased level of Nup98 is associated with the presence of bodies. Recruitment of the Y-complex and Elys into GLFG bodies requires the C-terminal domain of Nup98. During cell division, Y-Nup-containing GLFG bodies are disassembled in mitotic prophase, significantly ahead of nuclear pore disassembly. FRAP studies revealed that, unlike at nuclear pores, the Y-complex shuttles into and out of GLFG bodies. Finally, we show that within the nucleoplasm, a fraction of Nup107, a key component of the Y-complex, displays reduced mobility, suggesting interaction with other nuclear components. Together our data uncover a previously neglected intranuclear pool of the Y-complex that may underscore a yet-uncharacterized function of these nucleoporins inside the nucleus, even in cells that contain no detectable GLFG bodies.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Nucleus / metabolism*
  • Cell Nucleus / physiology
  • DNA-Binding Proteins / metabolism
  • Fluorescence Recovery After Photobleaching
  • HeLa Cells
  • Humans
  • Mitosis
  • Nuclear Pore Complex Proteins / metabolism*
  • Nuclear Proteins / metabolism*
  • Transcription Factors / metabolism

Substances

  • AHCTF1 protein, human
  • DNA-Binding Proteins
  • NUP107 protein, human
  • NUP160 protein, human
  • Nuclear Pore Complex Proteins
  • Nuclear Proteins
  • Transcription Factors