The motorized RhoGAP myosin IXb (Myo9b) in leukocytes regulates experimental autoimmune encephalomyelitis induction and recovery

Zhijun Liu; Yan Xu; Xueli Zhang; Jian Song; Lydia Sorokin; Martin Bähler

doi:10.1016/j.jneuroim.2015.03.014

The motorized RhoGAP myosin IXb (Myo9b) in leukocytes regulates experimental autoimmune encephalomyelitis induction and recovery

J Neuroimmunol. 2015 May 15:282:25-32. doi: 10.1016/j.jneuroim.2015.03.014. Epub 2015 Mar 14.

Authors

Zhijun Liu¹, Yan Xu¹, Xueli Zhang², Jian Song², Lydia Sorokin², Martin Bähler³

Affiliations

¹ Institute of Molecular Cell Biology, Westfalian Wilhelms-University Münster, Germany.
² Institute of Physiological Chemistry and Pathobiochemistry, Westfalian Wilhelms-University Münster, Germany.
³ Institute of Molecular Cell Biology, Westfalian Wilhelms-University Münster, Germany. Electronic address: baehler@uni-muenster.de.

PMID: 25903725
DOI: 10.1016/j.jneuroim.2015.03.014

Abstract

Myo9b regulates leukocyte migration by controlling RhoA signaling. Here we assessed its role in active experimental autoimmune encephalomyelitis (EAE). Myo9b(-/-) mice show a delay in the onset of EAE symptoms. The delay in disease onset was accompanied by reduced numbers of Th1 and Th17 cells in the CNS. Myo9b(-/-) mice showed no recovery from disease symptoms and exhibited elevated numbers of both Th17 cells and CD11b+ macrophages. Bone marrow chimeric mice demonstrated that the absence of a leukocyte source of Myo9b was responsible for the delayed leukocyte infiltration into the CNS, delayed EAE onset and lack of recovery.

Keywords: Bone marrow chimeric mice; Clinical features; Experimental autoimmune encephalomyelitis; Myosin IXb; RhoGAP.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD / metabolism
Cell Proliferation / genetics
Central Nervous System / metabolism
Central Nervous System / pathology
Cytokines / genetics
Cytokines / metabolism
Disease Models, Animal
Encephalomyelitis, Autoimmune, Experimental / chemically induced
Encephalomyelitis, Autoimmune, Experimental / pathology*
Encephalomyelitis, Autoimmune, Experimental / physiopathology*
Flow Cytometry
Gene Expression Regulation / genetics*
Image Cytometry
Leukocyte Common Antigens / genetics
Leukocyte Common Antigens / metabolism
Leukocytes / metabolism*
Mice
Mice, Inbred C57BL
Mice, Transgenic
Myelin-Oligodendrocyte Glycoprotein / toxicity
Myosins / genetics
Myosins / metabolism*
Peptide Fragments / toxicity
Recovery of Function / genetics*
Time Factors

Substances

Antigens, CD
Cytokines
Myelin-Oligodendrocyte Glycoprotein
Peptide Fragments
myelin oligodendrocyte glycoprotein (35-55)
myosin IXB
Leukocyte Common Antigens
Ptprc protein, mouse
Myosins