Analysis of cDNA and genomic clones coding for the pro alpha 1 chain of calf type II collagen

Nucleic Acids Res. 1985 Apr 25;13(8):2815-26. doi: 10.1093/nar/13.8.2815.

Abstract

A bovine cDNA library constructed from fetal cartilage RNA was screened with a pro alpha 1(II) collagen specific chicken cDNA. A recombinant clone (Bc 7), with an insert of 1 kb, was identified and shown to contain sequences exhibiting 85% homology with the chicken pro alpha 1(II) collagen C-propeptide. Interspecies comparison strongly suggested that one potential glycosylation site present in the avian C-propeptide is not utilized, since this site is absent in the bovine chain. In addition, two overlapping genomic clones (Pal 3 and Pal 4) were isolated and partially characterized. These clones span 23 kb of DNA and contain approximately 17 kb of the pro alpha 1(II) calf gene. Sequencing of exon 1 has determined the length of the 3' untranslated region and the exact location of the polyadenylation attachment site.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cartilage / analysis
  • Cattle
  • Chickens
  • Cloning, Molecular
  • Collagen / genetics*
  • DNA / analysis*
  • DNA Restriction Enzymes / metabolism
  • Nucleic Acid Hybridization
  • Poly A / metabolism
  • RNA / metabolism
  • RNA, Messenger

Substances

  • RNA, Messenger
  • Poly A
  • RNA
  • Collagen
  • DNA
  • DNA Restriction Enzymes

Associated data

  • GENBANK/X02420