Expression of human olfactory receptor 10J5 in heart aorta, coronary artery, and endothelial cells and its functional role in angiogenesis

Biochem Biophys Res Commun. 2015 May 1;460(2):404-8. doi: 10.1016/j.bbrc.2015.03.046. Epub 2015 Mar 17.

Abstract

ORs are ectopically expressed in non-chemosensory tissues including muscle, kidney, and keratinocytes; however, their physiological roles are largely unknown. We found that human olfactory receptor 10J5 (OR10J5) is expressed in the human aorta, coronary artery, and umbilical vein endothelial cells (HUVEC). Lyral induces Ca(2+) and phosphorylation of AKT in HUVEC. A knockdown study showed the inhibition of the lyral-induced Ca(2+) and the phosphorylation AKT and implied that these processes are mediated by OR10J5. In addition, lyral enhanced migration of HUVEC, which were also inhibited by RNAi in a migration assay. In addition, matrigel plug assay showed that lyral enhanced angiogenesis in vivo. Together these data demonstrate the physiological role of OR10J5 in angiogenesis and represent roles of ORs in HUVEC cells.

Keywords: AKT; Angiogenesis; Calcium; HUVEC; OR10J5; Olfactory receptor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aorta / enzymology
  • Aorta / metabolism*
  • Calcium / metabolism
  • Coronary Vessels / enzymology
  • Coronary Vessels / metabolism*
  • Endothelium, Vascular / enzymology
  • Endothelium, Vascular / metabolism*
  • Extracellular Signal-Regulated MAP Kinases / metabolism
  • Gene Knockdown Techniques
  • Human Umbilical Vein Endothelial Cells
  • Humans
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Neovascularization, Physiologic / physiology*
  • Phosphorylation
  • Proto-Oncogene Proteins c-akt / metabolism
  • Receptors, Odorant / metabolism*
  • Receptors, Odorant / physiology

Substances

  • OR10J5 protein, human
  • Receptors, Odorant
  • Proto-Oncogene Proteins c-akt
  • Extracellular Signal-Regulated MAP Kinases
  • Calcium