F-box protein Fbxo3 targets Smurf1 ubiquitin ligase for ubiquitination and degradation

Biochem Biophys Res Commun. 2015 Mar 20;458(4):941-5. doi: 10.1016/j.bbrc.2015.02.089. Epub 2015 Feb 24.

Abstract

It has been demonstrated previously that F-box protein Fbxl15 targets HECT-type E3 Smurf1 and forms a functionally active SCF complex for ubiquitination and proteasomal degradation. Here we show that another F-box protein Fbxo3, belonging to the FBXO type protein family, also interacts with and targets Smurf1 for poly-ubiquitination and proteasomal degradation. Different from Fbxl15, Fbxo3 targets all the Nedd4 family members for their degradation, indicating that Fbxo3 plays an important role in controlling the stability of Nedd4. Taken together, we show that Smurf1 is an endogenous substrate of Fbxo3. Our study gains further insight into the novel role of Fbxo3 in BMP signaling.

Keywords: Fbxo3; Proteasomal degradation; Protein ubiquitination; Smurf1; Ubiquitin ligase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Morphogenetic Proteins / metabolism
  • F-Box Proteins / metabolism*
  • HEK293 Cells
  • Humans
  • Proteasome Endopeptidase Complex / metabolism
  • Protein Interaction Maps
  • Proteolysis*
  • Ubiquitin-Protein Ligases / metabolism*
  • Ubiquitination*

Substances

  • Bone Morphogenetic Proteins
  • F-Box Proteins
  • Fbxo3 protein, human
  • SMURF1 protein, human
  • Ubiquitin-Protein Ligases
  • Proteasome Endopeptidase Complex