Abstract
Given its tumor-specific expression, including liver cancer, OY-TES-1 is a potential molecular marker for the diagnosis and immunotherapy of liver cancers. However, investigations of the mechanisms and the role of OY-TES-1 in liver cancer are rare. In the present study, based on a comprehensive bioinformatic analysis combined with RNA interference (RNAi) and oligonucleotide microarray, we report for the first time that downregulation of OY-TES-1 resulted in significant changes in expression of NANOG, CD9, CCND2 and CDCA3 in the liver cancer cell line BEL-7404. NANOG, CD9, CCND2 and CDCA3 may be involved in cell proliferation, migration, invasion and apoptosis, yet also may be functionally related to each other and OY-TES-1. Among these molecules, we identified that NANOG, containing a Kazal-2 binding motif and homeobox, may be the most likely candidate protein interacting with OY-TES-1 in liver cancer. Thus, the present study may provide important information for further investigation of the roles of OY-TES-1 in liver cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Apoptosis
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Carcinoma, Hepatocellular / genetics*
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Carcinoma, Hepatocellular / pathology
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / physiology*
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Cell Adhesion
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Cell Cycle Proteins / biosynthesis
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Cell Cycle Proteins / genetics
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Cell Cycle Proteins / physiology
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Cell Division
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Cell Movement
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Computational Biology
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Cyclin D2 / biosynthesis
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Cyclin D2 / genetics
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Cyclin D2 / physiology*
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Databases, Genetic
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Gene Expression Regulation, Neoplastic*
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Homeodomain Proteins / biosynthesis
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Homeodomain Proteins / genetics
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Homeodomain Proteins / physiology*
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Humans
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Liver Neoplasms / genetics*
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Liver Neoplasms / pathology
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Nanog Homeobox Protein
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / chemistry
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Neoplasm Proteins / genetics
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Neoplasm Proteins / physiology*
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Oligonucleotide Array Sequence Analysis
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Protein Interaction Mapping
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Protein Structure, Tertiary
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RNA, Bacterial
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RNA, Messenger / biosynthesis
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RNA, Neoplasm / biosynthesis
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Tetraspanin 29 / biosynthesis
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Tetraspanin 29 / genetics
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Tetraspanin 29 / physiology*
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Transcriptome
Substances
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ACRBP protein, human
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CCND2 protein, human
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CD9 protein, human
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CDCA3 protein, human
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Carrier Proteins
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Cell Cycle Proteins
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Cyclin D2
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Homeodomain Proteins
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NANOG protein, human
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Nanog Homeobox Protein
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Neoplasm Proteins
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RNA I
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RNA, Bacterial
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RNA, Messenger
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RNA, Neoplasm
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Tetraspanin 29