Abstract
The molecular complexes containing BCL10, MALT1 and CARMA proteins (CBM complex) have been recently identified as a key component in the signal transduction pathways that regulate activation of Nuclear Factor kappaB (NF-κB) transcription factor. Herein we identified the DEP domain-containing protein DEPDC7 as cellular binding partners of CARMA2 and CARMA3 proteins. DEPDC7 displays a cytosolic distribution and its expression induces NF-κB activation. Conversely, shRNA-mediated abrogation of DEPDC7 results in impaired NF-κB activation following G protein-coupled receptors stimulation, or stimuli that require CARMA2 and CARMA3, but not CARMA1. Thus, this study identifies DEPDC7 as a CARMA interacting molecule, and provides evidence that DEPDC7 may be required to specifically convey on the CBM complex signals coming from activated G protein-coupled receptors.
MeSH terms
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Adaptor Proteins, Signal Transducing / chemistry
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Blood Proteins / chemistry
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CARD Signaling Adaptor Proteins / immunology*
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CARD Signaling Adaptor Proteins / metabolism
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Dishevelled Proteins
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Gene Expression Regulation
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Guanylate Cyclase / immunology*
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Guanylate Cyclase / metabolism
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HEK293 Cells
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Humans
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Intracellular Signaling Peptides and Proteins / chemistry
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Intracellular Signaling Peptides and Proteins / immunology*
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Intracellular Signaling Peptides and Proteins / metabolism
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Membrane Proteins / immunology*
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Membrane Proteins / metabolism
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NF-kappa B / immunology*
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Phosphoproteins / chemistry
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Protein Binding
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Protein Structure, Tertiary
Substances
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Adaptor Proteins, Signal Transducing
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Blood Proteins
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CARD Signaling Adaptor Proteins
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CARD10 protein, human
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DEPDC7 protein, human
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Dishevelled Proteins
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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NF-kappa B
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Phosphoproteins
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platelet protein P47
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CARD14 protein, human
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Guanylate Cyclase
Grants and funding
The authors have no support or funding to report.