The Dishevelled, EGL-10 and pleckstrin (DEP) domain-containing protein DEPDC7 binds to CARMA2 and CARMA3 proteins, and regulates NF-κB activation

PLoS One. 2014 Dec 26;9(12):e116062. doi: 10.1371/journal.pone.0116062. eCollection 2014.

Abstract

The molecular complexes containing BCL10, MALT1 and CARMA proteins (CBM complex) have been recently identified as a key component in the signal transduction pathways that regulate activation of Nuclear Factor kappaB (NF-κB) transcription factor. Herein we identified the DEP domain-containing protein DEPDC7 as cellular binding partners of CARMA2 and CARMA3 proteins. DEPDC7 displays a cytosolic distribution and its expression induces NF-κB activation. Conversely, shRNA-mediated abrogation of DEPDC7 results in impaired NF-κB activation following G protein-coupled receptors stimulation, or stimuli that require CARMA2 and CARMA3, but not CARMA1. Thus, this study identifies DEPDC7 as a CARMA interacting molecule, and provides evidence that DEPDC7 may be required to specifically convey on the CBM complex signals coming from activated G protein-coupled receptors.

MeSH terms

  • Adaptor Proteins, Signal Transducing / chemistry
  • Blood Proteins / chemistry
  • CARD Signaling Adaptor Proteins / immunology*
  • CARD Signaling Adaptor Proteins / metabolism
  • Dishevelled Proteins
  • Gene Expression Regulation
  • Guanylate Cyclase / immunology*
  • Guanylate Cyclase / metabolism
  • HEK293 Cells
  • Humans
  • Intracellular Signaling Peptides and Proteins / chemistry
  • Intracellular Signaling Peptides and Proteins / immunology*
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Membrane Proteins / immunology*
  • Membrane Proteins / metabolism
  • NF-kappa B / immunology*
  • Phosphoproteins / chemistry
  • Protein Binding
  • Protein Structure, Tertiary

Substances

  • Adaptor Proteins, Signal Transducing
  • Blood Proteins
  • CARD Signaling Adaptor Proteins
  • CARD10 protein, human
  • DEPDC7 protein, human
  • Dishevelled Proteins
  • Intracellular Signaling Peptides and Proteins
  • Membrane Proteins
  • NF-kappa B
  • Phosphoproteins
  • platelet protein P47
  • CARD14 protein, human
  • Guanylate Cyclase

Grants and funding

The authors have no support or funding to report.