Preparation and characterization of human ADCK3, a putative atypical kinase

Protein Expr Purif. 2015 Apr:108:13-17. doi: 10.1016/j.pep.2014.12.008. Epub 2014 Dec 22.

Abstract

AarF domain containing kinase 3 (ADCK3) is a mitochondrial protein known to have a role in the electron transport chain. Despite being required for the biosynthesis of coenzyme Q10, a lipid-soluble electron transporter found to be essential for aerobic cellular respiration, the precise biological function of ADCK3 remains unknown. Patients with mutations in ADCK3 experience an onset of neurological disorders from childhood, including cerebellar ataxia and exercise intolerance. After extensive screening for soluble recombinant protein expression, an N-terminal fusion of maltose-binding protein was found to facilitate the overexpression of the human ADCK3 kinase domain in Escherichia coli as a soluble and biologically active entity. For the first time our work reveals Mg(2+)-dependent ATPase activity of ADCK3, providing strong support for the theoretical prediction of this protein being a functional atypical kinase.

Keywords: ADCK3; ATPase; Atypical kinase; Coenzyme Q(10) deficiency; Fusion protein.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Escherichia coli / genetics
  • Escherichia coli / metabolism
  • Humans
  • Mitochondrial Proteins* / biosynthesis
  • Mitochondrial Proteins* / chemistry
  • Mitochondrial Proteins* / genetics
  • Phosphotransferases* / biosynthesis
  • Phosphotransferases* / chemistry
  • Phosphotransferases* / genetics
  • Protein Structure, Tertiary
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics

Substances

  • COQ8A protein, human
  • Mitochondrial Proteins
  • Recombinant Proteins
  • Phosphotransferases