Abstract
The herpes simplex virus-1 DNA polymerase is a heterodimer of Mr 190,000 which consists of the products of the UL30 (Pol) and UL42 genes. The 136-kilodalton Pol gene product contains an intrinsic ribonuclease H activity that specifically degrades RNA.DNA heteroduplexes or duplex DNA substrates in the 5'----3' direction. It can therefore catalyze the excision of the RNA primers that initiate the synthesis of Okazaki fragments at a replication fork during herpes DNA replication.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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DNA-Directed DNA Polymerase / genetics
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DNA-Directed DNA Polymerase / isolation & purification
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DNA-Directed DNA Polymerase / metabolism*
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Endoribonucleases / metabolism*
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Exodeoxyribonuclease V
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Exodeoxyribonucleases / metabolism*
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Kinetics
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Molecular Weight
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Ribonuclease H
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Simplexvirus / enzymology*
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Simplexvirus / genetics
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Substrate Specificity
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Vero Cells
Substances
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DNA-Directed DNA Polymerase
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Endoribonucleases
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Exodeoxyribonucleases
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Exodeoxyribonuclease V
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Ribonuclease H