Levels of DNA topoisomerases, single-stranded-DNA-binding protein, and DNA polymerase I in rho+ and rho-15 strains of Escherichia coli

J Bacteriol. 1989 Sep;171(9):5183-6. doi: 10.1128/jb.171.9.5183-5186.1989.

Abstract

The Escherichia coli rho-15 mutant, which is highly defective in transcription termination, was examined to see whether its reduced DNA superhelicity could be explained by altered expression of proteins that may affect DNA structure. Levels of DNA gyrase and topoisomerase I were normal; levels of single-stranded-DNA-binding protein, DNA polymerase I, and a protein tentatively identified as Lon were significantly altered.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • DNA Polymerase I / metabolism*
  • DNA Topoisomerases, Type I / metabolism*
  • DNA Topoisomerases, Type II / metabolism*
  • DNA, Superhelical / genetics
  • DNA-Binding Proteins / metabolism*
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism
  • Rho Factor / genetics*
  • Terminator Regions, Genetic
  • Transcription Factors / genetics*
  • Transcription, Genetic

Substances

  • DNA, Superhelical
  • DNA-Binding Proteins
  • Rho Factor
  • Transcription Factors
  • DNA Polymerase I
  • DNA Topoisomerases, Type I
  • DNA Topoisomerases, Type II