A novel primase-free form of murine DNA polymerase alpha induced by infection with minute virus of mice

Biochemistry. 1989 May 30;28(11):4622-8. doi: 10.1021/bi00437a017.

Abstract

Two species of DNA polymerase alpha free of primase activity were identified in extracts of Ehrlich mouse cells that had been infected with minute virus of mice. Primase-free forms of DNA polymerase alpha eluted with 150 and 180 mM NaCl during ion-exchange chromatography on DEAE-cellulose columns, exhibited sedimentation coefficients of 11 S and 8.2 S, respectively, and were inhibited by aphidicolin, N2-(p-n-butylphenyl)-9-(2-deoxy-beta-D-ribofuranosyl)guanine 5'-triphosphate, and 2-(p-n-butylanilino)-9-(2-deoxy-beta-D-ribofuranosyl)adenine 5'-triphosphate. The ratio of primase-free DNA polymerase alpha to the DNA polymerase alpha-primase complex increased from 1.5 to greater than 100 during the course of infection, and free primase was produced during the MVM replicative cycle.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphidicolin
  • Carcinoma, Ehrlich Tumor / enzymology*
  • Carcinoma, Ehrlich Tumor / microbiology
  • Cell Line
  • Chromatography, Ion Exchange / methods
  • DNA Polymerase II / genetics
  • DNA Polymerase II / isolation & purification*
  • DNA Primase
  • DNA, Viral / metabolism
  • Diterpenes / pharmacology
  • Enzyme Induction / drug effects
  • Mice
  • Minute Virus of Mice / genetics
  • Mutation
  • Parvoviridae Infections / enzymology*
  • Parvoviridae Infections / genetics
  • RNA Nucleotidyltransferases / analysis*
  • Virus Replication

Substances

  • DNA, Viral
  • Diterpenes
  • Aphidicolin
  • DNA Primase
  • RNA Nucleotidyltransferases
  • DNA Polymerase II