Abstract
Here we report that the H3K9 demethylase KDM3B represses transcription of the angiogenesis regulatory gene, ANGPT1. Negative regulation of ANGPT1 by KDM3B is independent of its Jumonji (JmjC) domain-mediated H3K9 demethylase activity. We demonstrate that KDM3B downregulates ANGPT1 via interaction with SMRT, and suggest that the repressor complex is formed at the promoter area of ANGPT1. Using MTT and wound healing assays, depletion of KDM3B was found to increase cell proliferation and cell motility, indicating that KDM3B has a role in angiogenesis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Angiopoietin-1 / genetics*
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Angiopoietin-1 / metabolism
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Cell Movement
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Cell Proliferation
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Co-Repressor Proteins / metabolism
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Gene Expression Regulation
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HCT116 Cells
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Histones / metabolism*
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Humans
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Jumonji Domain-Containing Histone Demethylases / metabolism*
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K562 Cells
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Lysine / metabolism*
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MCF-7 Cells
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Nuclear Receptor Co-Repressor 2 / metabolism
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Protein Binding
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Repressor Proteins / metabolism*
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Transcription, Genetic*
Substances
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ANGPT1 protein, human
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Angiopoietin-1
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Co-Repressor Proteins
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Histones
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NCOR2 protein, human
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Nuclear Receptor Co-Repressor 2
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Repressor Proteins
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Jumonji Domain-Containing Histone Demethylases
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KDM3B protein, human
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Lysine