Electrochemically driven drug metabolism via a CYP1A2-UGT1A10 bienzyme confined in a graphene nano-cage

Jusheng Lu; Yuanjian Zhang; Henan Li; Jiachao Yu; Songqin Liu

doi:10.1039/c4cc06200k

Electrochemically driven drug metabolism via a CYP1A2-UGT1A10 bienzyme confined in a graphene nano-cage

Chem Commun (Camb). 2014 Nov 21;50(90):13896-9. doi: 10.1039/c4cc06200k.

Authors

Jusheng Lu¹, Yuanjian Zhang, Henan Li, Jiachao Yu, Songqin Liu

Affiliation

¹ Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. liusq@seu.edu.cn.

PMID: 25264962
DOI: 10.1039/c4cc06200k

Abstract

A graphene nano-cage with regulatable space for the assembly of a cytochrome P450 1A2-UDP-glucuronosyltransferase 1A10 bienzyme complex has been constructed via a click reaction, and successfully used to study drug sequential metabolism using an electrochemically-driven method.

MeSH terms

Cytochrome P-450 CYP1A2 / chemistry
Cytochrome P-450 CYP1A2 / metabolism*
Electrochemical Techniques*
Glucuronosyltransferase / chemistry
Glucuronosyltransferase / metabolism*
Graphite / chemistry
Graphite / metabolism*
Humans
Nanostructures / chemistry*
Pharmaceutical Preparations / chemistry
Pharmaceutical Preparations / metabolism*

Substances

Pharmaceutical Preparations
Graphite
CYP1A2 protein, human
Cytochrome P-450 CYP1A2
bilirubin uridine-diphosphoglucuronosyl transferase 1A10
Glucuronosyltransferase