A comparison of snRNP-associated Sm-autoantigens: human N, rat N and human B/B'

Nucleic Acids Res. 1989 Feb 25;17(4):1733-43. doi: 10.1093/nar/17.4.1733.

Abstract

N is a tissue-specific, Sm-epitope bearing, snRNP-associated protein found predominantly in brain. The cDNA sequence encoding human N is compared to those for rat N and human B/B'. The amino acid sequences of human and rat N are 100% conserved. Although the amino acid sequences of N and B/B' are very similar to each other, B/B' contains 50 amino acids which are not present in N. On Northern blots the cDNAs encoding N and B/B' recognize two different RNA species. A comparison of the codon usage, as specified by the open reading frames of N and B/B' as well as results from Southern blots, show that N and B/B' are derived from different genes.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Autoantigens / genetics*
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cerebellum / metabolism
  • DNA / genetics
  • Genes
  • Humans
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Rats
  • Restriction Mapping
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / immunology
  • Ribonucleoproteins, Small Nuclear

Substances

  • Autoantigens
  • RNA, Messenger
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear
  • DNA