Characterization of zebrafish Pax1b and Pax9 in fin bud development

Xuemei Chen; Huizhe Huang; Hua Wang; Fengjin Guo; Xiaogang Du; Linqiang Ma; Liang Zhao; Zhuma Pan; Haibo Gui; Taixian Yuan; Xin Liu; Lin Song; Yiquan Wang; Junling He; Han Lei; Rui Gao

doi:10.1155/2014/309385

Characterization of zebrafish Pax1b and Pax9 in fin bud development

Biomed Res Int. 2014:2014:309385. doi: 10.1155/2014/309385. Epub 2014 Aug 13.

Authors

Xuemei Chen¹, Huizhe Huang², Hua Wang³, Fengjin Guo², Xiaogang Du⁴, Linqiang Ma², Liang Zhao², Zhuma Pan², Haibo Gui¹, Taixian Yuan², Xin Liu³, Lin Song³, Yiquan Wang³, Junling He⁴, Han Lei⁵, Rui Gao²

Affiliations

¹ Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China ; Emergency Department, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
² Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China.
³ School of Life Sciences, Xiamen University, South Xiang'an Road, Xiamen 361102, China.
⁴ Department of Nephrology, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.
⁵ Faculty of Basic Medical Sciences, Chongqing Medical University, Medical College Road 1, Chongqing 400016, China ; Department of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Youyi Road 1, Chongqing 400042, China.

Abstract

Both Pax1 and Pax9 belong to the important paired box gene family (PAX), which mainly participates in animal development and sclerotome differentiation. To date, the precise molecular mechanism and related signaling pathway of Pax1 remain unclear. In our study, microinjection of morpholino- (MO-) modified antisense oligonucleotides against pax1b induced pectoral fin bud defects. Furthermore, we demonstrate that the phenotypes caused by the knockdown of Pax1b in zebrafish could not be phenocopied by pax9 MO and could not be rescued by either Pax1a or Pax9 overexpression. We further find that Pax1b affects the expression of col2a1, Uncx4.1, Noggin3, and aggrecan, confirming the role of Pax1b in chondrocyte differentiation and bone maturation. Moreover, we identify an interaction between PAX1 and FOXO1 and find that the interaction was enhanced under hypoxia stress. Together, this evidence for cell death caused by pax1b knockdown provides new insight into the role of the Pax protein family in cell fate determination and tissue specification.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Fins / abnormalities
Animal Fins / embryology*
Animal Fins / metabolism*
Animals
Bone Development
Cell Death
Embryonic Development* / genetics
Forkhead Box Protein O1
Forkhead Transcription Factors / metabolism
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
HEK293 Cells
HeLa Cells
Humans
Morphogenesis
PAX9 Transcription Factor / antagonists & inhibitors
PAX9 Transcription Factor / genetics
PAX9 Transcription Factor / metabolism*
Paired Box Transcription Factors / genetics
Paired Box Transcription Factors / metabolism*
Phenotype
Protein Binding
Stress, Physiological
Tail / abnormalities
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish / metabolism*
Zebrafish Proteins / antagonists & inhibitors
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism*

Substances

Forkhead Box Protein O1
Forkhead Transcription Factors
PAX9 Transcription Factor
Paired Box Transcription Factors
Pax1b protein, zebrafish
Zebrafish Proteins
foxo1a protein, zebrafish
pax9 protein, zebrafish