MiR-195 affects cell migration and cell proliferation by down-regulating DIEXF in Hirschsprung's disease

BMC Gastroenterol. 2014 Jul 9:14:123. doi: 10.1186/1471-230X-14-123.

Abstract

Background: Hirschsprung's disease (HSCR) is the most common congenital gut motility disorder. We aimed to investigate the roles of miR-195 in the pathogenesis of HSCR.

Methods: In this study, we measured the expression levels of miRNA, mRNA, and protein in colon tissues from 78 patients with HSCR and 66 controls without HSCR. Transwell, Cell Counting Kit-8 (CCK-8) and flow cytometry assay were employed to detect the function role of miR-195 in vitro.

Results: Our results showed that expression levels of miR-195 from patients with HSCR were significantly higher than control group; along with aberrant lower expression levels of digestive-organ expansion factor (DIEXF) were tested. Increased level of miR-195 could suppress the level of DIEXF in cell, which induced the impairment of cell migration and proliferation.

Conclusions: Aberrant expression of miR-195 may involved in the pathogenesis of HSCR by down-regulated the level of DIEXF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Case-Control Studies
  • Cell Movement / genetics*
  • Cell Proliferation / genetics*
  • Colon / metabolism*
  • Down-Regulation
  • Female
  • Gene Expression Profiling
  • Hirschsprung Disease / genetics*
  • Hirschsprung Disease / metabolism
  • Humans
  • Infant
  • Male
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • MIRN195 microRNA, human
  • MicroRNAs