Composition of isolated synaptic boutons reveals the amounts of vesicle trafficking proteins

Science. 2014 May 30;344(6187):1023-8. doi: 10.1126/science.1252884.

Abstract

Synaptic vesicle recycling has long served as a model for the general mechanisms of cellular trafficking. We used an integrative approach, combining quantitative immunoblotting and mass spectrometry to determine protein numbers; electron microscopy to measure organelle numbers, sizes, and positions; and super-resolution fluorescence microscopy to localize the proteins. Using these data, we generated a three-dimensional model of an "average" synapse, displaying 300,000 proteins in atomic detail. The copy numbers of proteins involved in the same step of synaptic vesicle recycling correlated closely. In contrast, copy numbers varied over more than three orders of magnitude between steps, from about 150 copies for the endosomal fusion proteins to more than 20,000 for the exocytotic ones.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain / ultrastructure
  • Exocytosis
  • Imaging, Three-Dimensional
  • Immunoblotting / methods
  • Mass Spectrometry / methods
  • Microscopy, Electron / methods
  • Models, Neurological
  • Presynaptic Terminals / chemistry
  • Presynaptic Terminals / metabolism*
  • Presynaptic Terminals / ultrastructure
  • Protein Transport
  • Rats
  • Rats, Wistar
  • Synaptic Vesicles / chemistry
  • Synaptic Vesicles / metabolism*
  • Synaptosomes / chemistry
  • Synaptosomes / metabolism*
  • Synaptosomes / ultrastructure
  • Vesicular Transport Proteins / analysis
  • Vesicular Transport Proteins / metabolism*

Substances

  • Vesicular Transport Proteins