A role of Rab29 in the integrity of the trans-Golgi network and retrograde trafficking of mannose-6-phosphate receptor

Shicong Wang; Zexu Ma; Xiaohui Xu; Zhen Wang; Lixiang Sun; Yunhe Zhou; Xiaosi Lin; Wanjin Hong; Tuanlao Wang

doi:10.1371/journal.pone.0096242

A role of Rab29 in the integrity of the trans-Golgi network and retrograde trafficking of mannose-6-phosphate receptor

PLoS One. 2014 May 2;9(5):e96242. doi: 10.1371/journal.pone.0096242. eCollection 2014.

Authors

Shicong Wang¹, Zexu Ma¹, Xiaohui Xu¹, Zhen Wang¹, Lixiang Sun¹, Yunhe Zhou¹, Xiaosi Lin¹, Wanjin Hong², Tuanlao Wang¹

Affiliations

¹ School of Pharmaceutical Sciences, State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen, Fujian, China.
² School of Pharmaceutical Sciences, State Key Laboratory of Cellular Stress Biology, Xiamen University, Xiamen, Fujian, China; Institute of Molecular and Cell Biology, A*STAR (Agency for Science, Technology and Research), Singapore, Singapore.

Abstract

Rab29 (also referred as Rab7L1) is a novel Rab protein, and is recently demonstrated to regulate phagocytosis and traffic from the Golgi to the lysosome. However, its roles in membrane trafficking have not been investigated extensively. Our results in this study revealed that Rab29 is associated with the trans-Golgi network (TGN), and is essential for maintaining the integrity of the TGN, because inhibition of the activity of Rab29 or depletion of Rab29 resulted in fragmentation of the TGN marked by TGN46. Expression of the dominant negative form Rab29T21N or shRNA-Rab29 also altered the distribution of mannose-6-phosphate receptor (M6PR), and interrupted the retrograde trafficking of M6PR through monitoring the endocytosis of CD8-tagged calcium dependent M6PR (cdM6PR) or calcium independent M6PR (ciM6PR), but without significant effects on the anterograde trafficking of vesicular stomatitis virus G protein (VSV-G). Our results suggest that Rab29 is essential for the integrity of the TGN and participates in the retrograde trafficking of M6PRs.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Blotting, Western
Cell Line
Endoplasmic Reticulum / metabolism
Endosomes / metabolism*
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism
HeLa Cells
Humans
MCF-7 Cells
Membrane Glycoproteins / genetics
Membrane Glycoproteins / metabolism
Microscopy, Fluorescence
Molecular Sequence Data
Mutation
Protein Transport
RNA Interference
Receptor, IGF Type 2 / genetics
Receptor, IGF Type 2 / metabolism*
Sequence Homology, Amino Acid
Viral Envelope Proteins / genetics
Viral Envelope Proteins / metabolism
rab GTP-Binding Proteins / genetics
rab GTP-Binding Proteins / metabolism*
rab1 GTP-Binding Proteins / genetics
rab1 GTP-Binding Proteins / metabolism*
trans-Golgi Network / metabolism*

Substances

G protein, vesicular stomatitis virus
Membrane Glycoproteins
Rab29 protein, human
Receptor, IGF Type 2
Viral Envelope Proteins
Green Fluorescent Proteins
rab GTP-Binding Proteins
rab1 GTP-Binding Proteins

Grants and funding

This work was supported by National Natural Science Foundation of China (No. 31071176, No. 30971442 and No. 30928011); International S & T Cooperation Program of China (No. 2013DFG32730). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.