MicroRNA let-7a-3 gene methylation is associated with karyotyping, CEBPA promoter methylation, and survival in acute myeloid leukemia

Leuk Res. 2014 May;38(5):625-31. doi: 10.1016/j.leukres.2014.03.008. Epub 2014 Mar 19.

Abstract

Let-7a-3 transcribes the miRNA let-7a, of which the expression is dysregulated in cancer. We evaluated the significance of let-7a-3 gene methylation in patients with de novo acute myeloid leukemia (AML). Let-7a-3 was methylated in 81.1% (73/90), partially methylated in 12.2% (11/90), or unmethylated in 6.7% (6/90) of patients. Let-7a-3 methylation correlated with AML karyotyping and CCAAT/enhancer binding protein α (CEBPA) methylation. Kaplan-Meier survival analysis predicted that let-7a-3 hypermethylation correlated with better survival in AML with hypomethylated CEBPA or with hypomethylated CEBPA without the favorable karyotype. We conclude that let-7a-3 methylation is a positive prognosticator for AML patients with hypomethylated CEBPA.

Keywords: Acute myeloid leukemia; CCAAT/enhancer binding protein α; DNA methylation; miRNA let-7a-3.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • CCAAT-Enhancer-Binding Proteins / genetics*
  • Cell Line, Tumor
  • DNA Methylation*
  • Humans
  • Karyotyping
  • Leukemia, Myeloid, Acute / genetics*
  • Leukemia, Myeloid, Acute / mortality
  • MicroRNAs / genetics*
  • Middle Aged
  • Promoter Regions, Genetic*

Substances

  • CCAAT-Enhancer-Binding Proteins
  • CEBPA protein, human
  • MicroRNAs
  • mirnlet7 microRNA, human