Expression of SRC suppressed C kinase substrate in rat neural tissues during inflammation

Neurochem Res. 2014 Apr;39(4):748-57. doi: 10.1007/s11064-014-1265-7. Epub 2014 Mar 13.

Abstract

Src-suppressed C kinase substrate (SSeCKS), an in vivo and in vitro protein kinase C substrate, is a major lipopolysaccharide (LPS) response protein which markedly upregulated in several organs, including brain, lung, heart, kidney etc., indicating a possible role of SSeCKS in inflammatory process. However, the expression and biological function of SSeCKS during neuronal inflammation remains to be elucidated, so we established an inflammatory model injected with LPS to investigate the gene expression patterns of SSeCKS in neural tissues by using TaqMan quantitative real-time PCR and immunohistochemistry in rat. Real-time PCR showed that LPS stimulated the expression of SSeCKS mRNA in a dose- and time-dependent manner in sciatic nerves, spinal cords and dorsal root ganglions. Immunohistochemistry showed that SSeCKS colocalized with nerve fibers in sciatic nerve after LPS administration, but there was no colocalization between SSeCKS and Schwann cells. In addition, SSeCKS colocalized with neurons which existed in dorsal root ganglions and spinal cords. These findings indicated that SSeCKS might play some important roles in sciatic nerve fibers and neurons in spinal cords and dorsal root ganglions after LPS injection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • A Kinase Anchor Proteins / biosynthesis*
  • Animals
  • Cell Cycle Proteins / biosynthesis*
  • Cells, Cultured
  • Dose-Response Relationship, Drug
  • Gene Expression Regulation, Enzymologic*
  • Inflammation / enzymology
  • Inflammation / pathology
  • Male
  • Neurons / enzymology*
  • Neurons / pathology
  • Rats
  • Rats, Sprague-Dawley
  • Schwann Cells / enzymology*
  • Schwann Cells / pathology
  • Spinal Cord / enzymology
  • Spinal Cord / pathology

Substances

  • A Kinase Anchor Proteins
  • Akap12 protein, rat
  • Cell Cycle Proteins