7SK small nuclear ribonucleoprotein complex is recruited to the HIV-1 promoter via short viral transcripts

Taketoshi Mizutani; Aya Ishizaka; Yutaka Suzuki; Hideo Iba

doi:10.1016/j.febslet.2014.01.067

7SK small nuclear ribonucleoprotein complex is recruited to the HIV-1 promoter via short viral transcripts

FEBS Lett. 2014 May 2;588(9):1630-6. doi: 10.1016/j.febslet.2014.01.067. Epub 2014 Mar 6.

Authors

Taketoshi Mizutani¹, Aya Ishizaka², Yutaka Suzuki³, Hideo Iba⁴

Affiliations

¹ Division of Host-Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Laboratory of Basic Science, Institute of Microbial Chemistry;BIKAKEN, 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan; RNA and Biofunctions, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan. Electronic address: mizutanit@bikaken.or.jp.
² Division of Host-Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan; Laboratory of Basic Science, Institute of Microbial Chemistry;BIKAKEN, 3-14-23 Kamiosaki, Shinagawa-ku, Tokyo 141-0021, Japan.
³ Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5 Kashiwanoha, Kashiwa-shi, Chiba 277-8568, Japan.
⁴ Division of Host-Parasite Interaction, Department of Microbiology and Immunology, Institute of Medical Science, University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.

PMID: 24607481
DOI: 10.1016/j.febslet.2014.01.067

Abstract

In this study, we demonstrate that the 7SK small nuclear ribonucleoprotein (snRNP) complex is recruited to the HIV-1 promoter via newly-synthesized HIV-1 nascent transcripts (short transcripts) in an hnRNP A1-dependent manner and negatively regulates viral transcript elongation. Our deep-sequence analysis showed these short transcripts were mainly arrested at approximately +50 to +70 nucleotides from the transcriptional start site in the U1 cells, an HIV-1 latent model. TNF-α treatment promptly disrupted the 7SK snRNP complex on the nascent transcripts and viral elongated transcripts were increased. This report provides insight into how 7SK snRNP complex is recruited to HIV-1 promoter in the absence of Tat.

Keywords: Chromatin regulation; Molecular biology; RNA virus; Viral transcription; Virology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
Gene Expression Regulation, Viral
Gene Knockdown Techniques
HEK293 Cells
HIV-1 / genetics*
Heterogeneous Nuclear Ribonucleoprotein A1
Heterogeneous-Nuclear Ribonucleoprotein Group A-B / genetics
Heterogeneous-Nuclear Ribonucleoprotein Group A-B / metabolism
Humans
Inverted Repeat Sequences
Molecular Sequence Data
Promoter Regions, Genetic
RNA, Small Interfering / genetics
RNA, Viral / genetics*
RNA-Binding Proteins / metabolism
Ribonucleoproteins / genetics
Ribonucleoproteins / metabolism*
Transcription Factors
Tumor Necrosis Factor-alpha / physiology

Substances

HEXIM1 protein, human
Heterogeneous Nuclear Ribonucleoprotein A1
Heterogeneous-Nuclear Ribonucleoprotein Group A-B
Larp7 protein, human
RNA, Small Interfering
RNA, Viral
RNA-Binding Proteins
Ribonucleoproteins
Transcription Factors
Tumor Necrosis Factor-alpha