A cellular stress response (CSR) that interacts with NADPH-P450 reductase (NPR) is a new regulator of hypoxic response

Biochem Biophys Res Commun. 2014 Feb 28;445(1):43-7. doi: 10.1016/j.bbrc.2014.01.110. Epub 2014 Jan 31.

Abstract

NADPH-P450 reductase (NPR) was previously found to contribute to the hypoxic response of cells, but the mechanism was not clarified. In this study, we identified a cellular stress response (CSR) as a new factor interacting with NPR by a yeast two-hybrid system. Overexpression of CSR enhanced the induction of erythropoietin and hypoxia response element (HRE) activity under hypoxia in human hepatocarcinoma cell lines (Hep3B), while knockdown of CSR suppressed them. This new finding regarding the interaction of NPR with CSR provides insight into the function of NPR in hypoxic response.

Keywords: Cellular stress response; Hypoxic response; NADPH-P450 reductase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • Carcinoma, Hepatocellular / genetics
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cell Hypoxia
  • Cell Line, Tumor
  • Erythropoietin / genetics
  • Gene Expression Regulation, Neoplastic
  • HEK293 Cells
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism*
  • Humans
  • Liver Neoplasms / genetics
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • NADPH-Ferrihemoprotein Reductase / genetics
  • NADPH-Ferrihemoprotein Reductase / metabolism*
  • Protein Binding
  • RNA Interference
  • Response Elements / genetics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Scavenger Receptors, Class A / genetics
  • Scavenger Receptors, Class A / metabolism*
  • Two-Hybrid System Techniques

Substances

  • Heat-Shock Proteins
  • SCARA3 protein, human
  • Scavenger Receptors, Class A
  • Erythropoietin
  • NADPH-Ferrihemoprotein Reductase