Abstract
This study evaluates a novel assay for detecting rifampin resistance in clinical Mycobacterium ulcerans isolates. Although highly susceptible for PCR inhibitors in 50% of the samples tested, the assay was 100% M. ulcerans specific and yielded >98% analyzable sequences with a lower limit of detection of 100 to 200 copies of the target sequence.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Buruli Ulcer / microbiology
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Drug Resistance, Bacterial*
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Humans
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Microbial Sensitivity Tests / methods
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Molecular Diagnostic Techniques / methods*
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Mycobacterium ulcerans / drug effects*
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Mycobacterium ulcerans / isolation & purification
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Rifampin / pharmacology*
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Sensitivity and Specificity
Substances
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Anti-Bacterial Agents
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Rifampin