The Oct-1 transcription factor belongs to the family of highly conserved POU-domain proteins that participate in regulation of housekeeping and tissue-specific gene expression. Several isoforms of Oct-1 were described previously. We have found the new alternative promoter of Oct-1 gene and the corresponding mRNA Oct-1X. This mRNA encodes the alternative Oct-1 isoform that has truncated N-terminus comparing to isoforms Oct-1A and Oct-1L. We explored the abundance of this isoform in human tissues and cell lines and demonstrated that it is ubiquitously expressed but its expression strongly varies in different tissues. By co-transfection and double luciferase assay we have demonstrated that Oct-1X is the activator of housekeeping (histone H2B) and tissue-specific genes (B29) transcription.