Expression of the mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) in tobacco leaves leads to phosphate export

FEBS Lett. 2014 Jan 31;588(3):482-9. doi: 10.1016/j.febslet.2013.12.013. Epub 2013 Dec 25.

Abstract

Phosphate homeostasis in multicellular eukaryotes depends on both phosphate influx and efflux. The mammalian Xenotropic Polytropic Virus Receptor 1 (XPR1) shares homology to the Arabidopsis PHO1, a phosphate exporter expressed in roots. However, phosphate export activity of XPR1 has not yet been demonstrated in a heterologous system. Here, wedemonstrate that transient expression in tobacco leaves of XPR1-GFP leads to specific phosphate export. Like PHO1-GFP, XPR1-GFP is localized predominantly to the endomembrane system in tobacco cells. These results show that tobacco leaves are a good heterologous system to study the transport activity of members of the PHO1/XPR1 family.

Keywords: PHO1; Phosphate export; Tobacco; Transporter; XPR1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arabidopsis / genetics
  • Arabidopsis Proteins / genetics
  • Gene Expression Regulation, Plant
  • Homeostasis
  • Nicotiana / growth & development
  • Nicotiana / metabolism
  • Phosphates / metabolism*
  • Plant Leaves / genetics
  • Plant Leaves / metabolism*
  • Plant Roots
  • Plants, Genetically Modified
  • Receptors, G-Protein-Coupled / biosynthesis
  • Receptors, G-Protein-Coupled / genetics*
  • Receptors, Virus / biosynthesis
  • Receptors, Virus / genetics*
  • Xenotropic and Polytropic Retrovirus Receptor

Substances

  • Arabidopsis Proteins
  • Phosphates
  • Receptors, G-Protein-Coupled
  • Receptors, Virus
  • Xenotropic and Polytropic Retrovirus Receptor