Molecular cloning of human prostate specific antigen cDNA

FEBS Lett. 1987 Apr 20;214(2):317-22. doi: 10.1016/0014-5793(87)80078-9.

Abstract

A lambda gt11 clone encoding prostate specific antigen has been isolated from a human prostate cDNA library. The cDNA insert of 1415 nucleotides hybridizes specifically to a prostate mRNA species of 1.5 kb. The nucleotide sequence codes for part of a signal peptide, a short propiece and the mature protein of 237 amino acid residues. The Mr for the non-glycosylated protein was 26,089. One potential site for N-linked carbohydrate attachment was identified. The primary structure shows extensive homology with proteases of the kallikrein family.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antigens / genetics*
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics*
  • Humans
  • Male
  • Prostate / immunology
  • Prostate-Specific Antigen

Substances

  • Antigens
  • DNA
  • Prostate-Specific Antigen

Associated data

  • GENBANK/X05332