Abstract
Giant cell tumor (GCT) of bone is an aggressive skeletal tumor characterized by localized bone resorption. Matrix metalloproteinase-13 (MMP-13) is the principal proteinase expressed by the stromal cells of GCT (GCTSCs) and also considered to play a crucial role in formation of the osteolytic lesion in GCT. However, the exact mechanism of the regulation of MMP-13 expression in GCTSCs was unknown. In this study, we identified miR-126-5p was significantly downregulated in GCTSCs and affect osteoclast (OC) differentiation and bone resorption by repressing MMP-13 expression at the post-transcriptional level. Thus, our studies show that miR-126-5p plays an important physiological role in multinucleated giant cell formation and osteolytic lesion in GCT.
Keywords:
GCT; MMP-13; MiR-126-5p; Osteoclast differentiation; Osteolysis.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
3' Untranslated Regions / genetics
-
Animals
-
Base Sequence
-
Bone Neoplasms / enzymology
-
Bone Neoplasms / genetics
-
Bone Neoplasms / metabolism
-
Bone Neoplasms / pathology
-
Bone Resorption / enzymology
-
Bone Resorption / genetics
-
Bone Resorption / pathology*
-
Cell Differentiation / genetics*
-
Cell Line
-
Down-Regulation / genetics
-
Gene Expression Regulation, Neoplastic
-
Genetic Engineering
-
Giant Cell Tumor of Bone / enzymology*
-
Giant Cell Tumor of Bone / genetics
-
Giant Cell Tumor of Bone / metabolism
-
Giant Cell Tumor of Bone / pathology*
-
Giant Cells / metabolism
-
Giant Cells / pathology
-
Matrix Metalloproteinase 13 / genetics
-
Matrix Metalloproteinase 13 / metabolism*
-
Mice
-
Mice, Inbred C57BL
-
MicroRNAs / genetics
-
MicroRNAs / metabolism*
-
Molecular Sequence Data
-
Osteoclasts / enzymology
-
Osteoclasts / pathology*
-
Protein Binding / genetics
-
Stromal Cells / enzymology
-
Stromal Cells / pathology
Substances
-
3' Untranslated Regions
-
MIRN126 microRNA, human
-
MicroRNAs
-
MMP13 protein, human
-
Matrix Metalloproteinase 13