Structural characterization of reverse transcriptase and endonuclease polypeptides of the acquired immunodeficiency syndrome retrovirus

J Virol. 1986 Nov;60(2):771-5. doi: 10.1128/JVI.60.2.771-775.1986.

Abstract

Automated N-terminal microsequencing of immune affinity-purified acquired immunodeficiency syndrome retrovirus polypeptides from infected cells was used to locate the N termini of 64-, 51-, and 34-kilodalton (kDa) polypeptides within the pol open reading frame (ORF) of the proviral DNA. The 64- and 51-kDa proteins had identical N termini (Pro-Ile-Ser-Pro-IIe-Glu-Thr-Val-) positioned 156 residues from the beginning of the pol ORF. The N terminus of the 34-kDa pol gene product, Phe-Leu-Asp-Gly-Ile-Asp-Lys-, mapped 716 residues into the pol ORF. These polypeptides were absent in an RT-negative, CD4-negative, persistently infected cell line (8E5) carrying a single defective copy of a constitutively expressed, integrated proviral DNA.

MeSH terms

  • Amino Acid Sequence
  • Cell Line
  • Endonucleases* / genetics
  • Genes, Viral
  • HIV / enzymology*
  • HIV / genetics
  • Molecular Weight
  • RNA-Directed DNA Polymerase* / genetics

Substances

  • RNA-Directed DNA Polymerase
  • Endonucleases

Associated data

  • GENBANK/K03455
  • GENBANK/M14124
  • GENBANK/M14125