Prdm6 is essential for cardiovascular development in vivo

PLoS One. 2013 Nov 21;8(11):e81833. doi: 10.1371/journal.pone.0081833. eCollection 2013.

Abstract

Members of the PRDM protein family have been shown to play important roles during embryonic development. Previous in vitro and in situ analyses indicated a function of Prdm6 in cells of the vascular system. To reveal physiological functions of Prdm6, we generated conditional Prdm6-deficient mice. Complete deletion of Prdm6 results in embryonic lethality due to cardiovascular defects associated with aberrations in vascular patterning. However, smooth muscle cells could be regularly differentiated from Prdm6-deficient embryonic stem cells and vascular smooth muscle cells were present and proliferated normally in Prdm6-deficient embryos. Conditional deletion of Prdm6 in the smooth muscle cell lineage using a SM22-Cre driver line resulted in perinatal lethality due to hemorrhage in the lungs. We thus identified Prdm6 as a factor that is essential for the physiological control of cardiovascular development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Body Patterning
  • Cardiovascular System / embryology*
  • Cell Differentiation
  • Cell Proliferation
  • DNA Primers
  • Mice
  • Mice, Knockout
  • Muscle, Smooth / cytology
  • Neovascularization, Physiologic
  • Oligonucleotide Array Sequence Analysis
  • Polymerase Chain Reaction
  • Repressor Proteins / genetics
  • Repressor Proteins / physiology*

Substances

  • DNA Primers
  • PRDM6, protein, mouse
  • Repressor Proteins

Associated data

  • GEO/GPL4937
  • GEO/GSE9065

Grants and funding

Jürgen Ruland: Deutsche Forschungsgemeinschaft TRR54, "Growth and survival, plasticity and cellular interactivity of lymphatic neoplasies", (http://www.trr54.de/), Martin Dyer: Medical Research Council "Consequences of genomic instability in B cells", (http://www.mrc.ac.uk/index.htm), Johannes Beckers: German National Genome Research Network, NGFN 01GS0850, (http://www.ngfn.de/en/start.html), Olaf Gross: fund by the "Bavarian Ministry of Sciences, Research and the Arts" in the Framework of the Bavarian Molecular Biosystems Research Network, (http://biosysnet.jimdo.com/projekte/regul%C3%A4re-juniorgruppen/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.