Abstract
Members of the PRDM protein family have been shown to play important roles during embryonic development. Previous in vitro and in situ analyses indicated a function of Prdm6 in cells of the vascular system. To reveal physiological functions of Prdm6, we generated conditional Prdm6-deficient mice. Complete deletion of Prdm6 results in embryonic lethality due to cardiovascular defects associated with aberrations in vascular patterning. However, smooth muscle cells could be regularly differentiated from Prdm6-deficient embryonic stem cells and vascular smooth muscle cells were present and proliferated normally in Prdm6-deficient embryos. Conditional deletion of Prdm6 in the smooth muscle cell lineage using a SM22-Cre driver line resulted in perinatal lethality due to hemorrhage in the lungs. We thus identified Prdm6 as a factor that is essential for the physiological control of cardiovascular development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Base Sequence
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Blotting, Northern
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Blotting, Southern
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Body Patterning
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Cardiovascular System / embryology*
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Cell Differentiation
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Cell Proliferation
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DNA Primers
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Mice
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Mice, Knockout
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Muscle, Smooth / cytology
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Neovascularization, Physiologic
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Oligonucleotide Array Sequence Analysis
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Polymerase Chain Reaction
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Repressor Proteins / genetics
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Repressor Proteins / physiology*
Substances
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DNA Primers
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PRDM6, protein, mouse
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Repressor Proteins
Grants and funding
Jürgen Ruland: Deutsche Forschungsgemeinschaft TRR54, "Growth and survival, plasticity and cellular interactivity of lymphatic neoplasies", (
http://www.trr54.de/), Martin Dyer: Medical Research Council "Consequences of genomic instability in B cells", (
http://www.mrc.ac.uk/index.htm), Johannes Beckers: German National Genome Research Network, NGFN 01GS0850, (
http://www.ngfn.de/en/start.html), Olaf Gross: fund by the "Bavarian Ministry of Sciences, Research and the Arts" in the Framework of the Bavarian Molecular Biosystems Research Network, (
http://biosysnet.jimdo.com/projekte/regul%C3%A4re-juniorgruppen/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.